APE  Vol.10 No.3 , August 2020
Effects of Brisk Walking Combined with Green Tea Extract on the Aerobic Capacity and Physical Fitness Function in Overweight and Obese Men: A Randomized, Double-Blind, Placebo-Controlled Trial
Abstract: In the present study we investigated the effects of brisk walking combined with GTE ingestion on the physical fitness function and aerobic capacity of inactive overweight and obese men. Participants were randomly assigned to the GTE group (150 mg GTE/tablet, twice/day) or the placebo group. All subjects in the GTE group (n = 12) or placebo group (n = 12) took part in the 12-week intervention program, and were followed for 4 weeks post-intervention. All variables were measured at week 0, week 12 and week 16. We found a significant increase in the aerobic capacity in both groups (P < 0.05), and a significant change in the handgrip strength (HS) of the GTE group (P < 0.05). After 12 weeks, statistically significant differences were observed in the 8-foot up-and-go (8FUG); and sit and reach (SR) test results in both groups (P < 0.05), while there was no significant change in the one-leg standing with eyes closed results of either group. A down-ward trend was observed after 4 weeks of follow-up. Brisk walking combined with GTE was suggested to have positive effects on aerobic capacity, SR, and 8FUG in overweight and obese men and was more effective in improving HS than brisk walking alone; however, it had no effect on static balance.

1. Introduction

Obesity is not only a major risk factor for chronic diseases, including metabolic syndrome, types 2 diabetes and some cancers, but also reduce the physical fitness functions in daily life (balance, endurance, walking steps) and the lifespan (Hita-Contreras et al., 2013; Lai et al., 2008; Pataky et al., 2014). In addition, obesity is significantly correlated with disability in daily life activities (Forhan, 2009). Increased physical activity is an important component of lifestyle intervention for the management of obesity. Walking, especially brisk walking, has been described as a near perfect “exercise” and has been recommended by health organizations worldwide (Morris & Hardman, 1997; Nemoto et al., 2007). A systematic review and meta-analysis by Hanson et al. reported that walking had a beneficial effect on body control, VO2max, and aerobic capacity in individuals (Hanson & Jones, 2015). Garnier et al. mentioned that 16 weeks of brisk walking was more effective than baseline for increasing cardiorespiratory fitness in moderately obese postmenopausal women (Garnier et al., 2015). In addition, Willems Met & Cook (2018) reported that green tea combined with brisk walking was able to reduce the respiratory exchange ratio and enhanced fat oxidation in women.

Green tea is one of the most popular beverages in the world, especially in Asia. Green tea is rich in catechins, among which epigallocatechin-3-gallate (EGCG) is the most pharmacologically active (Namal Senanayake, 2013). A previous study reported that green tea catechins combined with treadmill running was able to increase the endurance performance in mice (Minegishi et al., 2018). Sae-Tan et al. (Sae-Tan et al., 2014) also showed that GTE combined with voluntary running exercise was more effective for increasing the expression of peroxisome proliferator-activated receptor in skeletal muscle in high-fat fed mice. In addition, Novozhilov et al. (Novozhilov et al., 2015) showed that GTE combined with aerobic exercise was able to improve the aerobic exercise capacity and antioxidant system of erythrocytes. However, the effects of GTE combined with brisk walking on aerobic capacity and physical fitness in overweight and obese men, especially those with under uncontrolled dietary conditions are unknown.

Thus, we hypothesized that GTE combined with brisk walking might increase the aerobic capacity and physical fitness function in overweight and obese men. Therefore, the present study aimed to examine the effects of GTE combined with brisk walking on the change in: 1) aerobic capacity and 2) the balance, strength and other physical fitness function after 12 weeks and 4-weeks follow-up in inactive overweight and obese men.

2. Methods

2.1. Study Design

This study is a randomized, double-blind, placebo-controlled trial. This study was approved by the Luohe Central Hospital of Clinical Research Ethics Committee (No. 2018-03-015-E01) and has been registered as a clinical trial (Chinese Clinical Trials Registry, ChiCTR1900025134).

2.2. Participants

Twenty-four overweight or obese non-smoking men who were overweight or obese, (age, 39.8 ± 8.8 years) volunteered for this study. According to the physical characteristics of Asian, the optimal BMI cut-off value is 24 kg/m2 for men (Zeng et al., 2014). Those who met the following inclusion criteria were then included: male; between 25 and 59 years of age, BMI between 24 and 32, No participation in any clinical program within the previous 3 months. None were regular consumers of green tea. Individuals with known or suspected cardiovascular disease, diabetes, mental illness, liver, renal disease, and nerve disease were excluded from the study. Participants read and signed an informed consent form that explained the potential risks of this study.

2.3. Supplement

GTE and placebo were purchased from Damingtang Company (Hangzhou, China). Each GTE tablet contained 150 mg of EGCG (95% pure), isomaltitol, and microcrystalline cellulose (MC), and each placebo tablet contained neutral ingredients, such as corn starch, MC and isomaltitol.

2.4. Intervention

Twenty-four participants were randomly divided into 2 groups: the GTE combined with brisk walking (GTE) group and the placebo combined with brisk walking (placebo) group. Participants were assigned to either the control or GTE group through stratified randomization according to their body mass index (BMI). All participants performed physical activity irregularly. Enrollment and assignment were carried out by the first author. However, the GTE or placebo assignment was performed by the third assistant. In addition, because studies have demonstrated that GTE is positively correlated with hepatotoxicity, we selected dose of 300 mg EGCG/day, which is considered to be a safe level to ingest (Dekant et al., 2017; Hu et al., 2018). All participants were asked to consume two GTE or placebo tablets daily: one tablet with breakfast and one with dinner. The study assistant supervised the participants who consumed the GTE or placebo by telephone. The GTE tablet contained 150 mg of EGCG, while the placebo tablet contained neutral ingredients such as corn starch, and isomaltitol. The GTE and placebo tablets were, identical in appearance, size and color.

The brisk walking intervention program was conducted for 12 weeks. This outdoor walking program was performed under the supervision of the first author and a research assistant. The walking program involved 12 weeks of brisk walking at an intensity of 65% - 80% of the maximum heart rate, which was monitored using a heart rate belt (Murtagh E. M. et al., 2002). Brisk walking was performed 4 times a week, with each session lasting 60 minutes. The warm-up and cool down phases lasted 5 minutes. Due to all subjects being overweight or obese men with physical inactivity, for the first 3 weeks (initiation phase), we set the intensity to 50% - 65% of the maximum heart rate for all participants and then gradually increased the intensity. The maximum HR was calculated using the equation HRmax = [220 - age]. All participants were fitted with a heart rate monitor (FS1, Polar, Finland).

2.5. Physical Fitness Function Testing

All participants visited the hospital 3 times: at baseline, at 12 weeks after the start, and at 16 weeks after the start (for the 4-week follow-up). Body weight was measured with a body composition analyser (GAIA KIKO, Korea). Handgrip strength (HS) was measured to the nearest 0.1 kg using an electronic hand scale (EH101, CAMRY, China). Sit-and-reach (SR), 8-foot up-and-go (8FUG), and eyes closed and one-leg-standing (ECOLS) were measured using relevant professional instruments in a hospital. The 6 minutes walking test (6MWT) was measured indoors in a 30-m hospital corridor. The participants were instructed to walk as far as they could as fast as possible in 6 minutes; encouragement was given every minute during the test period. All parameters were measure at the center laboratory, Luohe Central of Hospital.

2.6. Statistical Analyses

All values are presented as the mean and standard deviation. All statistical analyses were performed using the SPSS 20.0 software program (IBM SPSS Statistics, Armonk, USA). An intention-to-treat (ITT) efficacy analysis was performed in this study. We used an analysis of co-variance (ANCOVA), to examine the changes between the groups after the intervention program. After observing the normality, a parametric analysis was performed using a repeated measure analysis of variance (2 groups at 3 time-points [baseline, post-test and follow-up]). In all comparisons, P values of <0.05 were considered to indicate statistical significance.

3. Results

3.1. Participants and Baseline Characteristics

An analysis of the baseline characteristics of the participants showed no significant differences between the groups with regard to age, body weight, or BMI. Furthermore, there were no significant differences between the groups in the baseline 6MWT, 8FUG, HS, SR, and ECOLS results (Table 1).

3.2. The Between-Group ANCOVA

The changes between groups were analyzed by an ANCOVA, with the pre-intervention levels as covariates and post-intervention levels as dependent variables. According to the results of the post-treatment ANCOVA, the 6WMT, 8FUG, and SR results of the two groups did not differ to a statistically significant extent (Table 2).

3.3. Repeated Assessments

As shown in Table 3, no significant group, time, or group × time main effects were found in ECOLS. Significant time main effects were found in the 6MWT, 8FUG, HS, and SR results (Table 3). In the placebo group, there were significant changes in the 6MWT and SR values, and a down-ward trend was observed after 4 weeks of follow-up. In the GTE group, the 6MWT and SR values were significantly reduced, and a downward trend was observed after 4 weeks of follow-up. There was a significant reduction in the 8FUG values after the intervention program; these values increased after follow-up. A similar result was observed in the placebo group. There were no significant groups or group × time main effects in any of the measurements in the GTE or placebo groups.

Table 1. Subject characteristics at baseline.

No difference between groups was statistically significant. Values are expressed as mean ±SD. m: meters; s: seconds; kg: kilogram; cm: centimeter; 6MWT: 6 minutes walking test; 8FUG: 8-foot up-and-go; HS: Handgrip strength; SR: Sit-and-reach; ECOLS: eyes closed and one-leg-standing.

Table 2. Changes in the aerobic capacity and physical fitness after 12 weeks’ intervention.

Values are expressed as mean ± SD.

Table 3. Aerobic capacity and physical fitness analysis of the participants during the experimental and follow-up periods.

Values are expressed as mean ± SE. *, #,&P < 0.05; *P-value for within-group comparisons in pre-and-post period; #P-value for within-group comparisons in post and follow-up; &P-value for within-group comparisons in pre and follow-up.

The results of the measurements of the aerobic capacity and physical fitness function are shown in Table 3. Regarding the physical fitness data, the GTE group exhibited significantly increased 6MWT levels, a significant change in 6MWT also observed in the placebo group. The ECOLS parameters’ were not normally distributed (Shapiro-Wilk normality test: P < 0.01). A Wilcoxon test showed no significant differences between the groups or within the groups.

In the GTE group, the 8FUG value decreased from 4.54 ± 0.21 s to 4.27 ± 0.16 s (P < 0.01) after the 12-weeks intervention program, while HS and SR were increased from 45.7 ± 1.55 kg to 48.1 ± 1.35 kg (P < 0.01) and 1.0 ± 2.64 cm to 4.1 ± 2.33 cm (P < 0.01), respectively. In the placebo group, the 8FUG value was reduced from 4.27 ± 0.21 s to 3.86 ± 0.16 s (P < 0.01), and there was a significant change in the SR level (P < 0.05) after the 12-week intervention program; these values tended to increase after the 4-week follow-up period.

4. Discussion

In the present study, we found significant changes in the 6MWT values of both groups after the 12-weeks intervention program and a downward trend after 4 weeks of follow-up. Animal and human studies reported that the mechanism underlying the improvement in endurance capacity by GTE combined with aerobic exercise was an increase in fat oxidation and a glycogen-sparing effect (Murase et al., 2005; Sae-Tan et al., 2011). Minegishi et al. (Minegishi et al., 2018) reported that a dietary intake of 0.5% green tea catechins increased fatty acid oxidation in skeletal muscle and improved the endurance capacity in mice. In a human study, Ichinose et al. (Ichinose et al., 2011) reported a significant reduction in the respiratory exchange ratio during endurance training in healthy male adults after 10 weeks of consuming a supplement containing green tea catechins (572.8 mg/day), while there was no change in a placebo group. This indicated that green tea catechins combined with aerobic exercise could increase whole body fat utilization during exercise. The findings of this study were contrary to those of a previous study, which showed that the daily intake of 570 mg of tea catechins combined with twice week aerobic exercise significantly altered the aerobic capacity of healthy male adults (Ota et al., 2016). Taken together, these studies suggest that the daily ingestion of high-dose GTE may improve aerobic capacity in overweight and obese men. Therefore, the discrepant results between the present study and the conclusion of the previous studies may be due to the following: first, the present study used a dose of only 300 mg EGCG/day. The second important confounding factor may be the diet. We did not control the diet in the present study.

The present study found a significant increase in HS in the GTE group and a tendency toward an increase in the placebo group. Our study results were in line with the current double-blind controlled trials, which showed that a 10-week exercise program had a beneficial effect on muscle mass in overweight and obese women, even there was no significant difference between groups (Smith et al., 2010). The significant change in the GTE group might have been because the ingestion GTE improved muscle recovery after exercise. A study by Panza et al. (Panza et al., 2016) also showed that tea consumption and eccentric exercise was able to hasten the strength recovery for 24 h after exercise. Another study showed that tea catechins and walking had a significant effect on muscle strength and walking ability in elderly women (Kim et al., 2013). The results of our study might have been due to a positive effect of GTE on muscle strength.

The results of the present study showed significant differences in the 8FUG and SR values of both groups after the 12-week intervention program and a downtrend in the follow-up period. The 8FUG and SR values did not differ between the GTE and placebo groups to a statistically significant extent. These results were in line with those of previous studies, which reported that aerobic exercise was able to improve the physical fitness function in adults (Melam et al., 2016; Ribeiro-Alvares et al., 2018). In addition, in the present study, we did not find a significant change in the ECOLS value after the 12-week intervention program. Although GTE has a positive effect on the body composition, muscle strength, and endurance capacity, no studies have focused on physical fitness in humans. We used these variables to assess the effects on the physical fitness function of the participants in our study. The effects of GTE combined with brisk walking might have been due to an indirect impact on body structure and posture, especially in overweight and obese adults.

The present study was associated with several limitations. First, the sample size was relatively small. Second, we did not set a group of control the dietary patterns of the subjects during the intervention period. Third, an increased number of physiological parameters should be used in a future study. Amulti-arm trial with a larger study population should be conducted to confirm the results of the present study.

5. Conclusion

This study showed that the ingestion of GTE combined with brisk walking in inactive overweight and obese men for 12 weeks significantly increased their aerobic capacity and physical fitness, but no significant difference was observed between the groups. We found a significant effect on the HS in the GTE group, which was more effective than brisk walking alone. In comparison to the post-intervention values, a downtrend was observed after the follow-up period. Further studies on the effects of exercise intensity, dietary modification and the daily-dose of GTE are needed to clarify the effects of GTE combined with brisk walking on the aerobic capacity and physical fitness function in overweight and obese humans.


This study was supported by Kyushu University Japan and Luohe Central of Hospital China. We are very grateful to all volunteers who took part in this study.


This study was funded by the Kyushu University.

Authors’ Contributions

Tengfei Zhang conceived of this study, designed and coordinated this trial, performed statistical analysis, interpretation and wrote the initial draft of the manuscript; Si Chen performed the randomization and statistical manuscript; Ningxia Li provided medical oversight. Zhenqing Hou performed the data entry. Atsushi Saito provided scientific guidance for the design of the study.

Cite this paper: Zhang, T. , Li, N. , Chen, S. , Hou, Z. and Saito, A. (2020) Effects of Brisk Walking Combined with Green Tea Extract on the Aerobic Capacity and Physical Fitness Function in Overweight and Obese Men: A Randomized, Double-Blind, Placebo-Controlled Trial. Advances in Physical Education, 10, 207-216. doi: 10.4236/ape.2020.103018.

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