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 JBBS  Vol.10 No.5 , May 2020
Isoflavone Attenuates the Nuclear Transcription Factor Kappa B (NF-κB) Activation on MPP+-Induced Apoptosis of PC12 Cells
Abstract: Objective: To explore the underlying molecular mechanisms of cellular response to the challenge by 1-methyl-4-phenylpyridinium (MPP+)-induced apoptosis of PC12 cells, an in vitro cell model for Parkinson’s disease, and the effect of NF-κB activation on the protection of Parkinson’s disease by Isoflavone (I). Methods: PC12 cells were used to establish the cell model of Parkinson’s disease, and are divided into five groups: control group; MPP+ group; I (Isoflavone) + MPP+ group; I group; SN-50 + MPP+ group. The content of NF-κB in PC12 cells was determined by immunocytochemistry; The viability of PC12 cells after treated with cell-permeable NF-κB inhibitor SN-50 and cell viability were measured by MTT assay; the expression levels of NF-κB p65 in cytoplasm and nuclear fractions were evaluated by western blot analysis; the mRNA expression of NF-κB p65 was analyzed by in situ hybridization (ISH). Results: Compared with the control group, the protein of NF-κB p65 both in cytoplasm and in nuclei was significantly higher than in I + MPP+ and MPP+ groups; similarly, the mRNA expression level of NF-κB p65 gene was also significantly higher; moreover, the protein expression of NF-κB p65 was much lower in I group (P < 0.05). In addition, compared with the MPP+ group, the protein of NF-κB p65 was significantly lower in I + MPP+ group, the mRNA expression level of NF-κB p65 gene was also significantly lower, and the protein expression level of NF-κB p65 was much lower in I + MPP+ group (P < 0.05); however, there was no significant difference between control group and I + MPP+ group (P > 0.05). Conclusion: NF-κB activation is essential to MPP+-induced apoptosis in PC12 cells; but Isoflavone can inhibit the cell damage to some extent to execute its protective function, which may be involved in nigral neurodegeneration in patients with Parkinson’s disease.
Cite this paper: Cheng, W. , Huang, A. , Zhang, L. , Feng, D. , Sun, X. , Xu, H. , Sun, Q. and Li, X. (2020) Isoflavone Attenuates the Nuclear Transcription Factor Kappa B (NF-κB) Activation on MPP+-Induced Apoptosis of PC12 Cells. Journal of Behavioral and Brain Science, 10, 191-199. doi: 10.4236/jbbs.2020.105012.
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