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Biography
Prof. Gary Black

Prof. Gary W. Black

Department of Applied Sciences, Faculty of Health and Life Sciences

Northumbria University, UK


Email: gary.black@northumbria.ac.uk


Qualifications


1992 PhD, Biochemistry and Molecular Biology. Awarded by the Council for National Academic Awards, UK.

1988 BSc, Applied Biology. Awarded by the Council for National Academic Awards, UK.


Publications (Selected)

  1. Malik V, Zhang M, Dover LG, Northen JS, Flinn A, Perry JJ, Black GW. (2013) Sterol 3β-glucosyltransferase biocatalysts with a range of selectivities, including selectivity for testosterone. Molecular BioSystems 9, 2816-2822.
  2. Karabencheva T, Carlsson U, Balali-Mood K, Black GW, Christov CZ. (2013) Conformational Effects on the Circular Dichroism of Human Carbonic Anhydrase II: A Multilevel Computational Study PLoS ONE 8(2): e56874.
  3. Malik V, Black GW. (2012) Structural, functional and mutagenesis studies of UDP-glycosyltransferases. Advances in Protein Chemistry and Structural Biology 87, 87-115.
  4. Yang Q, Porter AJ, Zhang M, Harrington DJ, Black GW, Sutcliffe IC. (2012) The impact of pH and nutrient stress on the growth and survival of Streptococcus agalactiae. Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology 102, 277–287.
  5. Yang Q, Zhang M, Harrington DJ, Black GW, Sutcliffe IC. (2011) A proteomic investigation of Streptococcus agalactiae reveals that human serum induces the C protein β antigen and arginine deiminase. Microbes and Infection 13, 757-760.
  6. van Pelt S, Zhang M, Otten LG, Holt J, Sorokin DY, van Rantwijk F, Black GW, Perry JJ, Sheldon RA. (2011) Probing the Enantioselectivity of a Diverse Group of Purified Cobalt-Centred Nitrile Hydratases. Organic & Biomolecular Chemistry 9, 3011-9.
  7. Zainab H. Elmabrouk ZH, Vincent F, Zhang M, Smith NL, Turkenburg JP, Charnock SJ, Black GW, Taylor EJ. (2011) Crystal structures of a family 8 polysaccharide lyase reveal open and highly occluded substrate-binding cleft conformations. Proteins: Function, Structure and Bioiformatics 79, 965–974.
  8. Yang Q, Zhang M, Harrington DJ, Black GW, Sutcliffe IC. (2010) A proteomic investigation of Streptococcus agalactiae grown under conditions associated with neonatal exposure reveals the upregulation of the putative virulence factor C protein beta antigen. International Journal of Medical Microbiology 300, 331–337.
  9. Black GW, Gregson T, McPake CB, Perry JJ, Zhang M. (2010) Biotransformation of nitriles using the solvent-tolerant nitrile hydratase fromRhodopseudomonas palustris CGA009. Tetrahedron Letters 51, 1639-1641.
  10. Martinez-Fleites C, Smith NL, Turkenburg JP, Black GW, Taylor EJ. (2009) Structures of two truncated phage-tail hyaluronate lyases fromStreptococcus pyogenes serotype M1. Acta Crystallographica Section F-Structural Biology and crystallization Communications 65, 963-966.
  11. Lindsay A-M, Zhang M, Mitchell Z, Holden MTG, Waller AS, Sutcliffe IC, Black GW. (2009) The Streptococcus equi prophage-encoded protein SEQ2045 is a hyaluronan-specific hyaluronate lyase that is produced during equine infection. Microbiology-SGM 155, 443-449.
  12. Sutcliffe IC, Black GW, Harrington DJ. (2008) Bioinformatics insights into the biosynthesis of the Group B carbohydrate in Streptococcus agalactae.Microbiology-SGM 154, 1354-1363.
  13. Zhang M, McDonald FM, Sturrock SS, Charnock SJ, Humphery-Smith I, Black GW. (2007) Group A streptococcus cell-associated pathogenic proteins as revealed by growth in hyaluronic acid-enriched media. Proteomics 7, 1379-1390.
  14. Sheldon WL, Macauley MS, Taylor EJ, Charnock SJ, Davies GJ, Vocadlo DJ, Black GW. (2006) Functional analysis of a group A streptococcal glycoside hydrolase from family 84 reveals it is a beta-N-acetylglucosaminidase and not a hyaluronidase. Biochemical Journal 399, 241-247
  15. Dennis RJ, Taylor EJ, Macauley MS, Stubbs KA, Turkenburg JP, Hart SJ, Black GW, Vocadlo DJ, Davies GJ. (2006) Structure and mechanism of a bacterial beta-glucosaminidase having O-GlcNAcase activity. Nature Structural & Molecular Biology 13, 365-371.
  16. Smith NL, Taylor EJ, Lindsay A-M, Charnock SJ, Turkenburg JP, Dodson EJ, Davies GJ, Black GW. (2005) Structure of a group A streptococcal phage-encoded virulence factor reveals a catalytically-active triple-stranded β-helix. Proceedings of the National Academy of Sciences (USA) 102, 17652-17657.
  17. O'Mahoney R, Doran J, Coffey L, Cahill OJ, Black GW, O'Reilly C. (2005) Characterisation of the nitrile hydratase gene clusters of Rhodococcus erythropolis strains AJ270 and AJ300 and Microbacterium sp. AJ115 indicates horizontal gene transfer and reveals an insertion of IS1166.Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology. 87, 221-32.
  18. Taylor EJ, Smith NL, Colby J, Charnock SJ, Black GW (2004). The gene encoding the ribulose monophosphate pathway enzyme, 3-hexulose-6-phosphate synthase, from Aminomonas aminovorus C2A1 is adjacent to coding sequences that exhibit similarity to histidine biosynthesis enzymes.Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology 86, 167-172.
  19. Humphry DR, Black GW, Cummings SP. (2003) Reclassification of'Pseudomonas fluorescens subsp. cellulosa' NCIMB 10462 (Ueda et al. 1952) as Cellvibrio japonicus sp. nov. and revival of Cellvibrio vulgaris sp. nov., nom. rev. and Cellvibrio fulvus sp. nov., nom. rev. International Journal of Systematic and Evolutionary Microbiology 53, 393-400.
  20. Charnock SJ, Brown IE, Turkenburg JP, Black GW, Davies GJ. (2002) Convergent evolution sheds light on the anti-b-elimination mechanism common to family 1 and 10 polysaccharide lyases. Proceedings of the National Academy of Sciences (USA) 99, 12067-12072.
  21. Czjzek M, Bolam DN, Mosbah A, Allouch J, Fontes CMGA, Ferreira LMA, Bornet O, Zamboni V, Darbon H, Smith NL, Black GW, Henrissat B, Gilbert HJ. (2001) The location of the ligand binding site of carbohydrate binding modules that have evolved from a common sequence is not conserved.Journal of Biological Chemistry 276, 48580-48587.
  22. Taylor EJ, Charnock SJ, Colby J, Davies GJ, Black GW. (2001) Cloning, purification and characterization of the 6-phospho-3-hexulose isomerase YckF from Bacillus subtilis. Acta Crystallographica D57, 1138-1140.
  23. Charnock SJ, Brown IE, Turkenburg JP, Black GW, Davies GJ. (2001) Characterization of a novel pectate lyase, Pel10A, from Pseudomonas cellulosa. Acta Crystallographica D57, 1141-1143.
  24. Humphry DR, George A, Black GW, Cummings SP. (2001) Flavobacterium frigidarium sp nov., an aerobic, psychrophilic, xylanolytic and laminarinolytic bacterium from Antarctica. International Journal of Systematic and Evolutionary Microbiology 51, 1235-1243.
  25. Brown IE, Mallen MH, Charnock SJ, Davies GJ, Black GW. (2001) Pectate lyase 10A from Pseudomonas cellulosa is a modular enzyme containing a family 2a carbohydrate-binding module. Biochemical Journal 355, 155-165.
  26. Colby J, Snell D, Black GW. (2000) Immobilization of Rhodococcus AJ270 and use of entrapped biocatalyst for the production of acrylic acid.Chemistry Monthly 131, 655-666.
  27. Cummings SP, Black GW. (1999) Polymer hydrolysis in a cold climate.Extremophiles 3, 81-87.
  28. Braithwaite KL, Barna T, Spurway TD, Charnock SJ, Black GW, Hughes N, Lakey JH, Virden R, Hazlewood GP, Henrissat B, Gilbert HJ. (1997) Evidence that galactanase A from Pseudomonas fluorescens subspecies cellulosa is a retaining family 53 glycosyl hydrolase in which E161 and E270 are the catalytic residues. Biochemistry 36, 15489-15500.
  29. Black GW, Rixon JE, Clarke JH, Hazlewood GP, Ferreira LMA, Bolam DN, Gilbert HJ. (1997) Cellulose binding domains and linker sequences potentiate the activity of hemicellulases against complex substrates. Journal of Biotechnology 57, 59-69.
  30. McKie VA, Black GW, MillwardSadler SJ, Hazlewood GP, Laurie JI, Gilbert HJ. (1997) Arabinanase A from Pseudomonas fluorescens subsp cellulosa exhibits both an endo- and an exo- mode of action. Biochemical Journal 323, 547-555.
  31. Black GW, Rixon JE, Clarke JH, Hazlewood GP, Theodorou MK, Morris P, Gilbert HJ. (1996) Evidence that linker sequences and cellulose-binding domains enhance the activity of hemicellulases against complex substrates.Biochemical Journal 319, 515-520.
  32. Millward-Sadler SJ, Hall J, Black GW, Hazlewood GP, Gilbert HJ. (1996) Evidence that the Piromyces gene family encoding endo-1,4-mannanases arose through gene duplication. FEMS Microbiology Letters 141, 183-188.
  33. Fanutti C, Ponyi T, Black GW, Hazlewood GP, Gilbert HJ. (1995) The conserved noncatalytic 40-residue sequence in cellulases and hemicellulases from anaerobic fungi functions as a protein docking domain. Journal of Biological Chemistry 270, 29314-29322.
  34. Millward-Sadler SJ, Davidson K, Hazlewood GP, Black GW, Gilbert HJ, Clarke JH. (1995) Novel cellulose-binding domains, nodB homologs and conserved modular architecture in xylanases from the aerobic soil bacteriaPseudomonas fluorescens subsp cellulosa and cellvibrio mixtus. Biochemical Journal 312, 39-48.
  35. Hall J, Black GW, Ferreira LMA, Millward-Sadler SJ, Ali BRS, Hazlewood GP, Gilbert HJ. (1995) The noncatalytic cellulose-binding domain of a novel cellulase from Pseudomonas fluorescens subsp. cellulosa is important for the efficient hydrolysis of avicel. Biochemical Journal 309, 749-756.
  36. Black GW, Hazlewood GP, Millward-Sadler SJ, Laurie JI, Gilbert HJ. (1995) A modular xylanase containing a novel noncatalytic xylan-specific binding domain. Biochemical Journal 307, 191-195
  37. Braithwaite KL, Black GW, Hazlewood GP, Ali BRS, Gilbert HJ. (1995) A non-modular endo-b-1,4-mannanase from Pseudomonas fluorescens subspeciescellulosa. Biochemical Journal 305, 1005-1010.
  38. Ali BRS, Zhou LQ, Graves FM, Freedman RB, Black GW, Gilbert HJ, Hazlewood GP. (1995) Cellulases and hemicellulases of the anaerobic fungusPiromyces constitute a multiprotein cellulose-binding complex and are encoded by multigene families. FEMS Microbiology Letters 125, 15-21.
  39. Pearson DM, O’Reilly C, Colby J, Black GW. (1994) DNA sequence of the cut a, cut b and cut c genes, encoding the molybdenum-containing hydroxylase carbon-monoxide dehydrogenase, from Pseudomonas thermocarboxydovorans strain C2. Biochimica et Biophysica Acta 1188, 432-438.
  40. Black GW, Hazlewood GP, Xue GP, Orpin CG, Gilbert HJ. (1994) Xylanase B from Neocallimastix patriciarum contains a noncatalytic 455-residue linker sequence comprised of 57 repeats of an octapeptide. Biochemical Journal299, 381-387.
  41. Zhou LQ, Xue GP, Orpin CG, Black GW, Gilbert HJ, Hazlewood GP (1994) Intronless celB from the anaerobic fungus Neocallimastix patriciarumencodes a modular family a-endoglucanase. Biochemical Journal 297, 359-364.
  42. Ferreira LMA, Wood TM, Williamson G, Faulds C, Hazlewood GP, Black GW, Gilbert HJ. (1993) A modular esterase from Pseudomonas fluorescens subspcellulosa contains a noncatalytic cellulose-binding domain. Biochemical Journal 294, 349-355.
  43. O’Reilly C, Black GW, Laffey R, McConnell DJ. (1990) Molecular analysis of an IS200 insertion in the gpt gene of Salmonella typhimurium LT2. Journal of Bacteriology 172, 6599-6601.
  44. Barstow DA, Black GW, Sharman AF, Scawen MD, Atkinson T, Li SS, Chia WN, Clarke AR, Holbrook JJ. (1990) Expression of the copy DNA for humana4 and b4 L-lactate dehydrogenases in Escherichia coli. Biochimica et Biophysica Acta 1087, 73-79.
  45. Black GW, Lyons CM, Williams E, Colby J, Kehoe M, O’Reilly C. (1990) Cloning and expression of the carbon-monoxide dehydrogenase genes fromPseudomonas thermocarboxydovorans strain C2. FEMS Microbiology Letters70, 249-254.