Dr. Steven N. Fiering

Dartmouth Medical School, USA



1990 Ph.D., Stanford University
1985 M.Sc., Eastern Michigan University

1974 B.Sc., University of Michigan

Publications (Selected)

  1. Hug, B. A., R. L. Wesselschmidt, S. Fiering, M. A. Bender, E. Epner, M. Groudine, T.J. Ley; Analysis of Mice Containing a Targeted Deletion of ß-globin Locus Control Region 5’ Hypersensitive Site 3; Molecular and Cellular Biology, 16, 2906-2912, 1996.
  2. Walters, M., W. Magis, S. Fiering, J. Eidemiller, D. Scalzo, M. Groudine; Transcriptional Enhancers Act to Suppress Position Effect Variegation in Cis; Genes and Development, 10, 185-196, 1996.
  3. Diecken, E., E.Epner, S. Fiering, K. Fournier, M. Groudine; Efficient Gene Targeting of the Human ß-globin in Recombination-Proficient Chicken/Human Microcell Hybrids; Nature Genetics, 12, 174-182, 1996.
  4. Magis, W., S. Fiering, M. Groudine, D. I. K. Martin; An Upstream Activator of transcription Coordinately Increases the Level and Epigenetic Stability of Gene Expression; Proceedings of the National Academy of Sciences USA, 93: 13914-13918, 1996.
  5. Zambrowicz, B. P., A. Imamoto, S. Fiering, L. A. Herzenberg, W. G. Kerr, P. Soriano; Disruption of Overlapping Transcripts in the ROSA ßgeo 26 Gene Trap Strain Leads to Widespread Expression of ß-galactosidase in Mouse Embryos and Hematopoietic Cells; Proceedings of the National Academy of Sciences USA, 94: 3789-3794, 1997.
  6. Garrick, D., S. Fiering, D. I. K. Martin, E. Whitelaw; Cremediated Reduction of Copies within a Transgene Array Provides Evidence for Repeat-induced Gene Silencing in Mammals; Nature Genetics 18:56-59, 1998.
  7. Seibler, J., D. Schubeler, S. Fiering, M. Groudine, J. Bode; DNA Cassette Exchange in ES cells Mediated by FLP Recombinase: An Efficient Strategy for Repeated Modification of Tagged Loci by Marker-free Constructs; Biochemistry, 37:6229-6234, 1998.
  8. Ley, T. J., B. Hug, S. Fiering, E. Epner,. M. A. Bender, M. Groudine; Reduced beta-globin gene expression in adult mice containing deletions of locus control region 5’ HS-2 or 5’ HS-3; Annals of the New York Academy of Sciences, 850:45-53, 1998.
  9. Epner, E, A. Reik, D. Cimbora, A. Telling, M. A. Bender, S. Fiering, T. Enver, D. I. K. Martin, M. Kennedy, G. Keller, M. Groudine; The ß-globin LCR is not necessary for an open chromatin structure or developmentally regulated transcription of the native mouse ß-globin locus; Molecular Cell 2:447-455, 1998.
  10. Bender, M. A., A. Reik, J. Close, A. Telling, E. Epner, S. Fiering, R. Hardison, M. Groudine; Description and targeted deletion of 5’ hypersensitive site 5 and 6 of the mouse ß-globin locus control region; Blood, 92:4394-4403, 1998.
  11. Howell, AL, T. N. Thacker, F. Li, S. Fiering, R. F. Graziano, J. Goldstein, and M. W. Fanger. Targeting HIV-1 gp120 to the high affinity Fc receptor (FcgRI, CD64) on myeloid antigen presenting cells: implications for enhancing vaccine responses. Current Topics in Virology 1:62-70, 1999.
  12. Feng Y-Q, J. Seibler, R. Alami, A. Eisen, K. A. Westerman, P. Leboulch, S. Fiering, E. Bouhassira; Site-specific chromosomal integration in mammalian cells: highly efficient CRE recombinase-mediated cassette exchange. Journal of Molecular Biology, 292: 779-785, 1999.
  13. Alami, R., J. M. Greally, K. Tanimoto, S. Hwang, Y.Q. Feng, J.D. Engel, S. Fiering, E. Bouhassira; The ß-globin LCR controls expression levels but does not block heterocellular position effects. Human Molecular Genetics 9:631-6, 2000.
  14. Alami, R., M. A. Bender, Y-Q. Feng, T. J. Ley, S. Fiering, M. Groudine, EE Bouhassira; Deletions within the mouse ß-globin LCR preferentially reduce ß-minor expression. Genomics 63:417-424, 2000.
  15. Bender, M. A., M.G. Mehaffey, A. Telling, B. Hug, T.J. Ley, M. Groudine, S. Fiering; Independent formation of DNaseI hypersensitive sites in the murine ß-globin locus control region. Blood, 95:3600-4, 2000.
  16. Feng, YQ, M. Lorincz, S. Fiering, J.M. Greally, E.E. Bouhassira;Position effects are influenced by the orientation of a transgene with respect to flanking chromatin. Molecular and Cellular Biology, 21, 298-309, 2001.
  17. Bender, MA, J. N. Roach, J. Halow, J. Close, R. Alami, E. E. Bouhassira, M. Groudine, S. Fiering; Targeted deletion of 5’HS1 and 5’HS4 of the ß-globin LCR reveals additive activity of DnaseI hypersensitive sites, Blood (plenary paper), 98, 2022-7, 2001
  18. Schneider, MJ, S. Fiering, S. E. Pallud, A.E. Parlow, D. L. St. Germain, V. A. Galton; Targeted disruption of the type 2 selenodeiodinase gene (DIO2) results in phenotype of pituitary resistance to thyroxine, Molecular Endocrinology, 15, 2137-48, 2001.
  19. Eszterhas, S, D. I. K. Martin, E.E. Bouhassira, S. Fiering; Transcriptional interference by independently regulated genes occurs in any relative arrangement of the genes and is influenced by chromosomal integration position, Molecular and Cellular Biology 22:469-79, 2002.
  20. Krieser, R.J., KS. MacLea, DS Longnecker, JL Fields, S. Fiering and A. Eastman. Deoxyribonuclease IIa is required for DNA digestion during the phagocytic phase of apoptosis and its loss causes perinatal lethalality, Cell Death Different., 9: 956-962, 2002.
  21. Hernandez, A., S. Fiering, E. Martinez, V.A. Galton, D. St.Germain;The gene locus encoding idothyroxine deiodinase type 3 (Dio3) is imprinted in the fetus and expresses antisense transcripts. Endocrinology, 143:4483-6, 2002.
  22. Hu, X., M. Bulger, J.N. Roach, S.K. Eszterhas, E. Olivier, E.E. Bouhassira, M. Groudine, S. Fiering; Promoters of the murine embryonic ß-like globin genes Ey and ßh1 do not compete for interaction with the ß-globin locus control region. Proceedings of the National Academy of Sciences USA 100:1111-5, 2003.
  23. Sutter, N.B., D. Scalzo, S. Fiering, M. Groudine, D. I. K. Martin; Chromatin insulation by a transcriptional activator. Proceedings of the National Academy of Sciences USA 100:1105-10, 2003.
  24. Rosenbauer, F., K. Wagner, J.L. Kutok, H. Iwasaki, M. LeBeau, Y. Okuno, K. Akashi, S. Fiering, D.G. Tenen, Acute myeloid leukemia induced by graded reduction of a lineage-specific transcription factor, PU. 1. Nature Genetics, 36: 624-30, 2004.
  25. Kato, KC, S. Martinez, P. Russel, Nurden, A. Nurden, S. Fiering, J. Ware; Genetic deletion of mouse platelet glycoprotein Ibbeta produces a Bernard-Soulier phenotype with increased alpha-granule size. Blood, 104: 2339-44, 2004.
  26. Kang, HC, JH Chae, YH Lee, M-A Park,JH Shin, S-H Kim, S-K Ye, YS Cho, S Fiering, CG Kim; Erythroid cell specific a-globin gene regulation by the CP2 transcription factor family. Molecular and Cellular Biology, 25:6005-20, 2005.
  27. Schneider, M., S. Fiering, B. Thai, S-Y Wu, E St. Germain, AF Parlow, DL St. Germain, VA Galton; Targeted disruption of the type 1 selenodeiodinase gene (Dio1) results in marked changes in thyroid hormone economy in mice. Endocrinology, 147:580-9, 2006.
  28. Hernandez, A, ME Martinez, S Fiering, VA Galton, D St. Germain: Type 3 deiodinase is critical for the maturation and function of the thyroid axis. Journal of Clinical Investigation, 116:476-84, 2006.
  29. Hu, X, M. Bulger, M. A. Bender, J. Fields, M. Groudine, S. Fiering: Deletion of the core region of 5’HS2 of the mouse ß-globin locus control region reveals a distinct effect in comparison with human ß-globin transgenes. Blood, 107:821-6, 2006.
  30. Hu, X, Eszterhas, S, Pallazzi, N, Bouhassira, EE, Fields, J, Tanabe, O, Gerber, SA. Bulger, M, Engel JD, Groudine, M, Fiering, S: Transcriptional Interference among the murine-like globin genes. Blood 109:2210-2216, 2007.
  31. Barre, L, C. Richardson, M. F. Hirshman, J. Brozinick, S. Fiering, B. E. Kemp, L. J. Goodyear,L.A. Witters A genetic model for the chronic activation of skeletal muscle AMP-activated protein kinase leads to glycogen accumulation.American Journal of Physiology: Endocrinology and Metabolism 292: E802-E811, 2007.
  32. Ma, Y, S. Fiering, C. Black, X. Liu, Z. Yuan, V. A. Memoli, D. J. Robbins, H. A. Bentley, G. J. Tsongalos, E. Dimidenko, S. J. Freemantle, E. Dmitrovsky. Transgenic Cyclin E triggers dysplasia and multiple pulmonary adenocarcinomas. Proceedings of the National Academy of Sciences USA, 104:4089-94, 2007.
  33. Hsu, Y. M., R. Mabaera, C. H. Lowrey, D. I. K. Martin, S. Fiering. CpG hypomethylation inlarge domain encompassing the embryonic eta-like globin genes in primitive erythrocytes. Molecular and Cellular Biology 27:5047-54, 2007.
  34. Srinivasulu, S., S. A. Acharya, M. Prabhakaran, M.E. Fabry, R. Alami, S. Fiering, E. E. Bouhassira, R.L. NagelHbS-Savaria: The antipolymerization effect of a single mutation in human alpha-chains. Protein Journal. 26: 523-32, 2007.
  35. Jain, S, Vinukonda, G, Fiering, SN, Kumar, A: A halotype of human angiotensinogen gene containing-217A increases blood pressure in transgenic mice as compared to 217G. American Journal of Physiology-Regulatory Integrative and Comparative Physiology, 295(6):R1849-57, 2008.
  36. Hsu, M, C. A. Richardson, E. Olivier, C. Qui, E. E. Bouhassira, C. H. Lowrey, S. Fiering: Complex developmental patterns of histone modifications associated with the human eta-globin switch in primary cells. Experimental Hematology. 37:799-806, 2009.
  37. Coon, C. I., S. Fiering, J. Gaudet, C.A. Wyatt, C. E. Brinckerhoff: Site controlled transgenic mice validating increased expression from human matrix metalloproteinase (MMP-1) promoter due to a naturally occurring SNP, Matrix Biology 28:425-31 (2009).
  38. Fromm, G., C. de Vries, R. Byron, J. Fields, S. Fiering, M. Groudine, M. A. Bender, J. Palis, and M. Bulger: Histone hyperacetylation within the beta-globin locus is context-dependent and precedes high-level gene expression. Blood, 114:3479-88 (2009).
  39. Guan, Z., C. M. Hughes, S. Kosiyatrukul, P. Norio, R. Sen, S. Fiering, C. D. Allis, E. E, Bouhassira, C. L. Schildkraut. Decreased replication origin activity in temporal transition regions. Journal of Cell Biology 187:623-35 (2009).
  40. Liu, X, L. F. Sempere, F. Galimberti, S.J. Freemantle, C. Black, K. H. Dragnev, Y. Ma, S. Fiering, V. Memoli. J. Li, S. Kauppinen E. Dmitrovsky: Uncovering Growth-Suppressive MicroRNAs in Lung Cancer. Clinical Cancer Research 15: 1177-83, (2009).
  41. Crane-Godreau, M, M.M. Maccani, S.K.Eszterhas, S.L. Warner, J.A. Jukosky, S. Fiering: Exposure to cigarette smoke disrupts CCL20-mediated antimicrobial activity in respiratory epithelial cells. Open Immunology Journal 2: 86-93, (2010).
  42. Lathrop, MJ, L Chakrabarti, J Eng, CH Rhodes, T. Lutz, A. Nieto, HD. Liggitt, S Warner, J. Fields, R. Stoger, S. Fiering: Deletion of the Chd6 exon 12 affects motor coordination. Mammalian Genome 3-4: 130-42 (2010).
  43. Akada, H, D. Yan, H. Zou, S. Fiering, RE Hutchinson, MG Mohi:Conditional expression of heterozygous or homozygous Jak2V617F from its endogenous promoter induces a polycythemia vera-like disease. Blood, 115: 3589-97, (2010).
  44. Berland, R., S. Fiering, H. Wortis: A conserved enhancer element differentially regulates developmental expression of CD5 in B and T cells. Journal of Immunology 185:7537-43, (2010).
  45. Jain, S., A. Tillinger, B. Mopidevi, VG Pandey, C Chauhan, S Fiering, S Warming, A. Kumar: Transgenic mice with -6A haplotype of the human angiotensinogen gene have increased blood pressure compared to -6G haplotype, Journal Biol. Chem, 285:41172-86, 2010.
  46. Fromm G, Cadiz-Rivera B, de Vries C, Getman M, McGrath KE, Kingsley PD, Fields J, Fiering S, Bulger M.An embryonic stage-specific enhancer within the murine {beta}-globin locus mediates domain-wide histone hyperacetylation. Blood 117; 52-7-14 2011.