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Biography
Prof. Barbara Pioselli

Biomolecules Structural Characterization Unit, head

Preclinical R&D Analytics and Early Formulations Department, Chiesi, Parma, Italy


Email: b.pioselli@chiesi.com


Qualifications

2004 Ph.D., Biochemical Sciences

2001 B.S., Pharmaceutical Chemistry


Research Fields

Protein Chemistry

Mass spectrometry

Protein structural and functional characterization

Enzymology


Publications (Selected)

  1. Raboni, S., Pioselli, B., Bettati, S. and Mozzarelli, A. (2003). The molecular pathway for the allosteric regulation of tryptophan synthase. Biochim. Biophys. Acta 1647, 157-160.
  2. Bettati, S., Pioselli, B., Campanini, B., Viappiani, C. and Mozzarelli, A. (2004). Protein-doped nanoporous silica gels. Encyclopedia of Nanoscience and Nanotechnology, Editor H. S. Nalwa, American Scientific Publishers.
  3. Pioselli, B., Bettati, S., Demidkina, T. V., Zakomirdina, L. N., Phillips, R. S., and Mozzarelli, A. (2004). Tyrosine phenol lyase and tryptophan indole-lyase encapsulated in wet nanoporous silica gels: selective stabilization of tertiary conformations. Protein Sci 13 913-924.
  4. Pioselli B, Bettati S, Mozzarelli A. (2005) Confinement and crowding effects on tryptophan synthase alpha2beta2 complex. FEBS Letters 579 2197-2202
  5. Ronda L, Pioselli B, Bruno S, Micalella C, Bettati S, Mozzarelli A.
    Biocatalysis in a confined environment: lessons from enzymes immobilized in wet, nanoporous silica gels (2007) Chimica oggi-Chemistry today 5 10-15
  6. Deitrich CL, Raab A, Pioselli B, Thomas-Oates JE, Feldmann J Chemical preparation of an isotopically enriched superoxide dismutase and its characterization as a standard for species-specific isotope dilution analysis. (2007) Anal Chem 79 8381-90
  7. Caccia D, Frassi R, Perrella M, Del Favero E, Ronda L, Pioselli B, Bettati S, Mozzarelli A. - PEGylation promotes hemoglobin tetramer dissociation. (2009) Bioconjug. Chem. 20 1356-1366.
  8. Raab A, Pioselli B, Munro CN, Thomas-Oates JE, Feldmann, J - Gel electrophoresis with laser ablation ICP-MS detection for metallo-proteins: a SWOT-Analysis. (2009) Electrophoresis, 30, 303-314.
  9. Pioselli B, Munro CN, Raab A, Feldmann J and Thomas-Oates JE - SOLUTION PHASE IEF for metal binding protein analysis. (2009) Metallomics, 1, 501-510.
  10. Deitrich CL, Raab A, Munro, CN, Pioselli B, Krupp, EM, Thomas-Oates JE, Feldmann J.- Absolute quantification of superoxide dismutase (SOD) using species-specific isotope dilution analysis. (2010) Anal. Bioanal.Chem. 397, 3515-3524
  11. Ronda L, Pioselli B, Bruno S, Faggiano S, Mozzarelli A. Electrophoretic analysis of PEGylated hemoglobin-based blood substitutes.(2011) Analytical Biochemistry 408 118-123
  12. Faggiano S, Bruno S, Ronda L, Pizzonia P, Pioselli B, Mozzarelli A Modulation of expression and polymerization of hemoglobin Polytaur, a potential blood substitute. (2011). Arch Biochem Biophys 505(1) 42-7.
  13. Micalella C, Martignon S, Bruno S, Pioselli B, Caglio R, Valetti F, Pessione E, Giunta C, Rizzi M. (2011). X-ray crystallography, mass spectrometry and single crystal microspectrophotometry: A multidisciplinary characterization of catechol 1,2 dioxygenase. Biochim Biophys Acta. 1814 (6) 817-823.
  14. Pioselli B, Paredi G, Mozzarelli A. (2011). Proteomic analysis of pork meat in the production of cooked ham. Mol. BioSystems 7, 2252-2260.
  15. Mozzarelli A., Paredi G., Pioselli B., Raboni S. (2012) From meat to food: the proteomics assessment. Farm animal proteomics. Proceedings of the 3rd Managing Committee Meeting and 2nd Meeting of Working Groups 1, 2 & 3 of COST Action FA1002.
  16. Campanini B., Pioselli B., Raboni S., Felici P., Giordano I., D’Alfonso L., Collini M., Chirico G., Bettati S. (2013) Role of histidine 148 in stability and dynamics of a highly fluorescent GFP variant. BBA Proteins and Proteomics, 2013 1834(4) 770-9..
  17. Zecchi R., Trevisani M., Pittelli G., Pedretti P., Manni ME., Pieraccini G., Pioselli B., Amadei F., Moneti G., Catinella S. Impact of drug administration route on drug delivery and distribution into the lung: an imaging mass spectrometry approach. (2013) European Journal of Mass Spectrometry, 19, 475-482.