Patients with chronic inflammatory diseases are increasing, particularly those associated with hyper responsive immune system, including asthma, inflammatory bowel disease (IBD), chronic fatigue syndrome, atherosclerosis and rheumatoid arthritis. The biggest pharmacological hurdles of today are to treat such chronic inflammatory disorders. Furthermore, diseases like multiple sclerosis, chronic asthma, rheumatoid arthritis, psoriasis, and inflammatory bowel disease, are strongly debilitating and are becoming progressively more common among aged people in our society. People affected worldwide with bones diseases like rheumatoid arthritis and osteoarthritis are the major victims of these inflammatory disorders. In this regard, the fourth leading cause of disability by the year 2020 would be osteoarthritis, in aging populations. Moreover, several epidemiological studies have shown inflammation as one of the major risk factors in emerging various kinds of neoplastic transformation  .
Inflammation is s part of the complex biological response of vascular tissues to harmful stimuli  . It is a protective reaction of body’s cells to injury or infections and allergic or chemical irritation. This is a reaction that is characterized by certain inflammatory features which are redness, pain, swelling, heat, and loss of function because of the blood vessels dilation that leads to the increase of blood flow in that area, thus resulting in the migration of immune cells like neutrophils and macrophages, along with the fluids causing edema toward the inflamed regions. The process of inflammation is quite complex, initiated by several factors which include molecules that ranges from bacteria to chemical and therefore results in cellular trauma or death. Tissue injury induced by this trauma results in the inflammatory mediators release including reactive oxygen species (ROS) like superoxide anion (O2−), hydrogen peroxide (H2O2), nitric oxide and cytokines  -  . Immune system disorders have been linked to increased expression of pro-inflammatory mediators, including cytokines, NADPH oxidase, NF kappa B, myeloperoxidase, and Inos  .
Pharmacological and physiological constituents of the herbal medicines are known to regulate and modulate various functions of inflammatory response in the body either directly or indirectly  . The highly complex immune system is however containing well organized set of cells and each cell in this group has a defined function which is particularly essential to protect the body from diseases. Immune cells may interact in a cell to cell manner and also act in response to intercellular messages during the transfer of hormones, and cytokines  . Peripheral immune system comprises of, lymphocytes leukocytes, mast cells, and platelets, whereas in central nervous system, the cells that amend inflammatory reactions are, microglial cells, endothelial cells and astrocytes     .
Major incidents of inflammatory reaction that trigger robust hyperactive immune response could be summarizing in following five categories. First: Nitric Oxide (NO) and prostaglandin synthesis  , second: NF kappa B expression, third: reactive oxygen species (ROS)  , fourth: migration of leukocytes  , and finally the fifth: is increased production of pro-inflammatory cytokines i.e. TNF, IL6 and IL1  .
2. Role of the Free Radicals in Pathology of Inflammation
Reactive oxygen and nitrogen species generated by macrophages and neutrophils Figure 1 upon encounter of an antigen or allergen have been shown its implication in immune system disorders . This phenomenon of generation of free radicals is known as oxidative burst which is accomplished by involvement of NADPH oxidase, present on the surface of neutrophils membrane. The NADPH oxidase which is reactive oxidant producing enzyme or by inducible nitric oxide synthase (iNOS) expressed in activated phagocytic cells both reactive nitrogen and reactive oxygen species are discussed in detail below  -  .
Figure 1. Free radical species generated during inflammation.
3. Nitric Oxide
Under normal conditions, Nitric oxide (NO) is known to participate in physiological processes, such as vasodilatation and neurotransmission, however, over expression of this molecule have been documented to lead to diseases like asthma, inflammation, atherosclerosis and organ transplant rejection. Many other factors such as the persistent inflammation of the stomach commonly caused by the pathogenic bacterium, Helicobacter pylori, chronic obstructive pulmonary disease and liver inflammation caused by smoking and alcohol consumption that leads to lung cancer and liver cirrhosis respectively. Therefore, tissue inflammation from gastritis, hepatitis, and colitis are all correlated with enhanced NO production. For instance, in inflammatory cells, the inducible nitric oxide synthase when activated, it induces iNOS activation in macrophages, hence cause persistence NO production. NO produced in this way shows toxicity to cells and damage to the surrounding tissue. Nevertheless, when NO produced by constitutive forms of NOS, proven essential to sustain the normal function of cells  -  .
During the inflammatory conditions, cell expresses iNOS, which is considered to be regulated primarily at the level of gene expression. Once expressed, iNOS is thought to constantly produce NO in presence of an adequate substrate as well as cofactors needed, until degradation of iNOS protein  .
These properties have led to the conclusion that iNOS generates NO in an unregulated fashion with mainly cytotoxic properties. Hence scientists start believing that nitric oxide (NO) is one of the major mediators which cause inflammation and cancers in several organs. For instance, the excessive production of this free radical become more toxic to the host tissue, when react with superoxide radicals which is directly damaging specie for the normal functions of cells. There are two other major forms of nitric oxide synthase NOs, the endothelial (eNOS) and the neuronal nitric oxide synthase (nNOS) which already known now beside the inflammatory iNOS. The iNOS (130 kDa) is the inducible form of enzyme, primarily found in macrophages as a homodimer under native conditions, However, for fully functional enzymatic activity depends upon tetrahydrobiopterin-dependent dimerization. In cases where inflammation continues over months or even years, the nearby cells may be exposed to considerable quantities of highly reactive chemical species eventually, leading to debilitating diseases      .
3.1. Cellular Activation
Toll Like Receptors are type I transmembrane receptors with a single membrane-spanning domain, and a leucine-rich extracellular ligand-binding domain that contains repeats of a non-polar amino acid leucine, and an intracellular Toll like receptor domain. There are number of human TLRs functioning either as homodimer or heterodimers, these receptors are known to be involved in recognition of particular set of pathogen-associated molecular patterns (PAMPs). For examples the LPS activate the TLR4. An array of an external stimulus has been recognized to activate distinctive signaling pathways that initiate expression of the proinflammatory iNOS   .
3.2. Signaling that Trigger the NO Release by Macrophages
Cell wall of Gram-negative bacteria comprise of good amount of LPS (Lipopolysaccharide), Figure 2 this LPS could serve as an initiator for inflammatory cascades. The LPS upon encounter to a cell, it interacts with LPS Binding Protein LBP, which in turn delivers LPS to CD14, the CD14-LPS complex with the help of MD2 (Lymphocyte antigen 96) interacts with TLR4, leading to initiation of signaling pathway via adaptors molecules that are MyD88 and IRAK p38, TRAF6, and TAB1 by these adaptors is done. Eventually TLR4 activation by LPS leads to NF-κB activation. NF-κB is pleotropic transcription factor which is
Figure 2. LPS induced signaling pathway.
present in almost all cell types and is involved in many biological processes such as inflammation, immunity cell growth differentiation, and tumorigenesis. The NF-κB is a homo or hetero dimer, compose of Rel like domain containing proteins, REL and NF-κB2/p52 and heterodimeric NF-κB p65 - p50 complexes appear to be one of the most abundant protein in the cell, the dimer binds at kappa B site in the DNA and act as transcriptional activator or repressor respectively. NF-κB complex is held in cytoplasm by in an inactive state complexed with member of NF-κB inhibitor I-κB family      .
In a conventional activation pathway, I-κB is phosphorylated by I-κB kinase (IKK) in response to different activator subsequently degraded thus liberating the active NF-κB complex which translocate to the nucleus, NF-κB-p65-p50 complex is a transcriptional activator it sits on κB elements in the iNOS 5' site, triggering iNOS transcription. Additionally, cytokines released from infected host cells, TNF-α and IL-1β also known to activate NO production The IFN-γ cytokine interacts with the IFNR1 and IFNR2 complex, and causes the activation of JAK/STAT pathways, leading to the synthesis of IRF1transcription factor and stimulation of iNOS mRNA transcription    .
The IFN-γ also found to provides a synergistic effect to the LPS induction of iNOS transcription because IRF1 interacts with NF-κB, altering the conformation of the NOS2 promoter. They enhances the binding of transcription factors, such as NF-κB and AP-1, by DNA-protein and protein-protein interactions  .
4. Reactive Oxygen Species (ROS)
NADPH oxidase is activated during inflammation, resulting in generation of superoxide (•O−2) and by action of superoxide dismutase (SOD), it gets connected to H2O2. Further chloride gets involve and makes H2O2 more toxic, in presence of myeloperoxidase, which usually activated by neutrophils and results in formation of very toxic HOCl, Figure 3. Therefore, the enzymes NADPH Oxidase is normally found in a resting state and function in redox signaling as second messenger. However, under abnormal conditions stimulated phagocytes involved in oxidative stress.
The NADPH oxidase complex is a cluster of proteins that donate an electron from NADPH to molecular oxygen (O2) to produce superoxide ( ). This initiates the respiratory burst, a key step in immune defense against bacterial and fungal pathogens. The importance of this process to human health is manifested in chronic granulomatous disease (CGD), which refers to any of several hereditary diseases in which certain oxidase proteins are defective. The result is a reduce superoxide production and impaired clearance of bacterial pathogens, leading to the formation of a granuloma, or fibrotic nodule, around the persistent bacterial infection. While CGD underscores the significance of NADPH oxidase in professional phagocytes, like neutrophils, monocytes, and macrophages, NADPH oxidase has additional roles in other cell types. These non-phagocytic versions of the NADPH oxidase produce less superoxide, which is involved predominantly in the inter- and intra-cellular signaling      .
Figure 3. Major pathways involved in the intracellular production of reactive oxygen/nitrogen species.
4.1. Cellular Activation
A variety of stimuli can lead to superoxide production through NADPH oxidase, but in the phagocytes, a very strong response is known be achieved through ligand that activates Gq-type GPCRs. Some of the molecules that activates specific Gq-coupled receptors include PAF, IL-8, various proteases, nucleotides like ATP, and N-formylated peptides (fMLP). Receptor activation causes Gq to initiate hydrolysis of membrane-associated phosphatidylinositol bisphosphate (PIP2) by phospholipase C β (PLCβ), giving rise to inositol trisphosphate (IP3) and diacylglycerol (DAG). The IP3 promotes the opening of calcium (Ca2+) channels in the endoplasmic reticulum (ER), leading to a transient rise in intracellular Ca2+ levels. It is an established fact that both Ca2+ and DAG activate PKC, which results in its movement from the cytoplasm to a membrane. Ca2+ also activates cytosolic PLA2 (cPLA2), which moves to the ER and perinuclear membranes to selectively liberate arachidonic acid (AA) stored in membrane phospholipids. The AA is known to act as a second messenger and is believed to regulate many neutrophil functions, although the underlying mechanisms and its physiologic role are poorly understood. Stimulation of intact neutrophils with exogenous AA leads to activation of PKCs, phosphatidylinositol 3-kinases (PI-3K), PLC, PLD, and mitogen-activated protein kinases (MAPK). Exogenous AA has long been known to activate neutrophil superoxide   .
4.2. Assembly of the NADPH Oxidase Complex
In the resting phagocytes, a portion of the oxidase is integrated in membranes while other components remain soluble in cytoplasm (Figure 4). The membrane-bound section consists of a large glycosylated protein, gp91phox, and a smaller
Figure 4. The NADPH oxidase complex.
adapter protein, p22phox, collectively referred to as cytochrome b558. The gp91phox protein contains two heme groups and binds the redox cofactor flavin adenine dinucleotide (FAD), suggesting that it is the workhorse of the oxidase. The GTPase rap1 is also sometimes described as associated with cytochrome b558, but this association, as well as the function of rap1, remains controversial. The p47phox, p67phox, and p40phox, proteins are found linked together by SH3 domains and SH3 binding sites. Cell stimulation through a Gq-coupled receptor drives PKC-mediated phosphorylation of p47phox on several residues, resulting in the translocation of this soluble complex to the bound complex at the membrane, with p47phox binding to p22phox through an SH3 domain. It is important to note that p47phox can be phosphorylated by several other kinases (e.g., Akt, MAPK, c-Src), which in turn are activated by distinct stimuli. Over a dozen sites on p47phox have been shown to be phosphorylated; the role(s) of each of these modifications are important areas of current research. However, it is clear that phosphorylation of p47phox alters its shape, enabling translocation and activity. The p67phox is absolutely essential for full oxidase activity and in transferring electrons from NADPH to FAD; it is phosphorylated on Thr233 during cell activation. The p40phox appears to serve a negative regulatory role within the NADPH oxidase complex, with phosphorylation on Thr154 affecting this role     .
4.3. The Trigger for ROS
Phorbol esters are among the most potent activators of the neutrophil respiratory burst, acting as analogs of diacylglycerol (DAG) and directly activating many members of the serine-threonine protein kinase C (PKC) family. The downstream effects of PKC include direct phosphorylation of p47phox, which further leads to membrane translocation of cytosolic components in a cell-free system and intact cells. Other activator like chemoattractant Formyl-Met-Leu-Phe (fMLP), immunoglobulin G (IgG)-opsonized zymosan or other bacteria processed by engulfing through receptors, coat the surface of professional phagocytes. Similarly, receptor binding initiates a cascade of signals that culminate in the cell membrane engulfing the bacterium in a vesicle, the phagosome. Along with the bacterium the membrane proteins will be internalized which includes cytochrome b558 (gp91/p22) and FAD; these and other membrane-bound proteins will also be delivered to the internalized phagosome as it gets matures. Receptor signaling activates kinases that phosphorylate soluble phox proteins to initiate assembly of the NADPH oxidase complex    .
The phagosome pocket is formed in response to antigen engulfment by phagocytes, where a series of vesicles fuse with the phagosome to aggressively destroy and take part the foreign pathogen. Granules from rapidly deliver pre-formed enzymes, include defensing, myeloperoxidase, gelatinases, and cathepsins, to the maturing phagosome, aiding in killing. During maturation of early to late endosomes soluble and membrane-bound proteins are delivered from the endoplasmic reticulum and Golgi to the phagosome. Finally, lysosomes infuse digestive enzymes that function in the acidic conditions of the mature phagosome, degrading the bacterium. The entire process of bacterial capturing, killing, and degradation can take place in time spam of less than 60 mints. In phagocytes which also act as antigen-presenting cells, portions of digested prey may be recirculated to the cell surface for presentation to lymphocytes to propagate the immune response   .
5. Pathogenesis of ROS/NO
During inflammation the inducible form of nitric oxide synthase becomes activated and causes the robust generation of NO that causes excessive vasodilation resulting in hypotension, and septic shock. This may result in fatal complications in older age, and in young people during bacterial infection leading to sepsis. One of the most toxic radical derived from NO is peroxynitrite (ONOO−) that is generated when NO combines with superoxide (ROS)-released during phagocytosis. The ONOO− generation, eventually reduces the bioavailability of basal NO and, therefore, affect its function on endothelial relaxation causing vasoconstriction, and hypertension, which may lead to atherosclerosis. On the other hand, excess formation of ONOO− causes nitrosylation of proteins, which contributes in cancer development. In addition to this, NO also plays a role in heart and lung diseases, septic shock, as well as in impotence. This wide role of NO in various pathological conditions prompted scientists to develop potent NO inhibitor      .
Reactive Nitrogen Species such as NO are involved in inflammation-induced carcinogenesis, as it known to induce guanine nitration, producing G:C to T:Atransversion. The products of nitric oxide synthesis induce mutations through N-nitrosation of secondary amines and may play a critical role in carcinogenesis induced during chronic inflammation because these N-nitrosamines are markedly mutagenic. Additionally, peroxynitrite (ONOO−) is very toxic and causes mutations through DNA strand break and DNA base modification . Furthermore, ROS/RNS can cause enhanced expression of proto oncogenes by DNA base modification, strand breaks, and tumor-suppressor genes disruption. Overall, oxidative stress has been shown to induce malignant transformation of cells in culture. Nevertheless, the progression of human cancer depends on other factors as well, including the extent of DNA damage, DNA repair systems functioning, and the cytotoxic effects of ROS in large amounts as well as their growth-promoting effects in small amounts. For example, the proliferative responses of Syrian hamster embryo fibroblasts to superoxide, and the low levels of O−2 can enhance cell growth, whereas constant unnecessary oxidative DNA damage may ignite signals (like p53 a tumor suppressor gene) and therefore induce apoptosis. In addition, ROS/RNS induced DNA strand breaks results in accumulation of p53 inside the nucleus, the cell growth at the G/S phase is arrested in order to allow DNA to repair its lesions before going in replication phase    .
5.1. Targeting iNOS
NO serve as neurotransmitter under stress conditions whenever, NO concentration increase causes the unnecessary vasodilation leads to hypotension. Different studies have shown that a series of potent and selective inducible nitric-oxide synthase (iNOS) inhibitors prevent dimerization of enzyme iNOS in cells, and inhibit iNOS in vivo. Then inhibitors could be a better therapeutic approach for the above mentioned diseases. However, it is also evident that most of the compound not directly inhibit enzyme, rather inhibition could be at mRNA level or through inhibition of the transcription factor NF-k B Inhibition of the transcription factor could be of therapeutic potential since its pathway is directly involved in chronic inflammatory diseases. Different target sites have been mention in Figure 5     .
5.2. Targeting NADPH Oxidase
In some inflammatory diseases scientist aim to target Activation of NADPH oxidases which may result from the stimulation of a number of cell surface receptors, such as the angiotensin II receptor, which is particularly important in hypertension and heart failure due to the complex mechanisms involved in the activation of NADPH oxidases, these enzymes can be targeted at several different levels of their activity. Firstly, decreasing NADPH oxidase expression can lead to inhibition. Also, the activation of NADPH oxidase can be decreased by blocking the translocation of its cytosolic subunits to the membrane. Another
Figure 5. Mechanism of nitric oxide inhibition. Shows the possible target sites in activated macrophages.
possibility is inhibition of the p47phox subunit, either by preventing its phosphorylation using PKC inhibitors, or by blocking its binding to other subunits. A decrease of signal transduction and inhibition of Rac 1 translocation have also been demonstrated to decrease ROS generation   .
NADPH oxidase is multisubunit membrane protein that is activated by translocation of the cytosolic subunits p47phox, p67phox, and Rac to the Nox/p22phox complex. Some of the inhibitors act by interfering with this translocation. Nonspecific inhibitors target the flavin-containing subunit (DPI), the major activators of the oxidase are the ACE inhibitors and angiotensin receptor blockers, whereas upstream kinases, the PKC inhibitors inhibit translocation of p47 subunit. Some inhibitors act as scavenger of the reactive oxygen species known as antioxidants   .
6. Anti Inflammatory Drugs in the Molecular Biology Era
Based on the cellular and molecular pathways involved in progression of inflammation, can be ameliorated and eventually treated with pure compounds pos. It is well known that anti-inflammatory properties of several natural compounds isolated from a verity of plants, e.g, flavonoids and its derivatives, phytosterol, genistein, tocopherol, curcumin ascorbic acid, and others are the widely used inhibitors of the molecular targets of pro-inflammatory mediators in inflammatory drug design research   .
Other plants that contain triterpenoids, alkaloids, saponins, tannin, and anthraquinones, have been reported to possess a diverse range of bioactivities which includes anticancer, antibacterial, immunomodulating, antimalarial, and anti-tuberculosis activities. Other studies with synthetic derivatives suggested that most of those derivative exhibit potential of anti-inflammatory property by blocking pro inflammatory mediators such as derivatives of thiazole, alkyl derivatives and Bergenin   .
Currently, a number of drugs in clinical uses possess antioxidant property as an example the Tamoxifen, is a drug of choice and is widely used for the cure of breast cancer it is found to exert antioxidant its effects in addition to the anti oestrogenic properties. It has been reported that it suppresses H2O2 production in human neutrophils. This drug is given as a prophylactic drug for breast cancer. Another example is the most commonly used drug sulphasalazine which is also found to act as a free-radical scavenger. Sulphasalazine and its metabolites are now used in treatment of IBD. When Sulphasalazine is administered, it gets converted by the colonic bacteria into 5-aminosalicylic acid (5-ASA), which is powerful antioxidant. It can efficiently scavenge free oxygen redials serving as excellent scavenger of HOCl. The tamoxifen metabolite 4-hydroxytamoxifen inhibitor of lipid peroxidation  .
7. Prevention from Oxidative Stress and Need Based Research
Overall, imbalance between antioxidant defense mechanism and oxygen-derived species generation in vivo leads to state of oxidative stress. There is evidently no great reserve of antioxidant defenses in mammals, perhaps because some oxygen-derived species may involve in metabolism. Certain compounds or strategies cause an activation of mitochondrial oxygen consumption and promote increased formation of ROS formation. These molecules culminating in increased stress resistance and longevity. During aging the oxidative stress of the organism is increasing and approaches to lower the increased ROS formation in our cells should be implemented. The most efficient preventive step to avoid exogenous free-radical exposure would be to avoid, exogenous toxic molecules cigarette smoke, pollutants and UVR, since it’s not always possible, protection could be obtained by adequate antioxidant protection, decreasing the formation of free radicals, or increasing damage-repair systems of the cells  .
Paradoxically, the efficiency of defense and repair may be enhanced by different measures caloric restriction (with adequate vitamin and mineral intake) for the prolonging of life. On the other hand, the reduction of energy metabolism may actually reduce ROS generation from mitochondria and consequently extend lifespan. In either case, avoiding electron leakage from electron transport and the resultant ROS production seem to be essential for a normal life. In order to reduce endogenous oxidative stress lifestyle approach to be followed. Consumption of vegetables and plant-derived foods and beverages has positive effect on the prevention of age associated diseases like coronary heart disease and atherosclerosis as well as for longevity. Avoiding mental stress, meditation and limit intake of fats and sugar is another way of preventing from oxidative stress. Besides that, after consuming a meal, perform work instead of resting should in order to maintain an appropriate electron flow. The regulation of energy by the body’s circadian rhythms also plays a significant role in controlling oxidative stress    .
In addition to this if person suffering from inflammation must to cure using the medication however the medications may limit the symptoms but could not provide a complete healing. With advent of NSAIDs physicians treated successfully Rheumatoid Arthritis patients, unfortunately later developed gastrointestinal bleeding, because of long term administration of aspirin along with cortisone. Since that time, the pharmaceutical industry and researchers are trying to find solution and new ways to overcome the gastrointestinal toxicity caused by this effective drug of choice which is combination of steroids plus NSAIDs. Recently researcher is investigating anti-inflammatory entities that are immunomodulating which possess inhibitory activity against oxidative stress particularly with specific targeted molecule. Eventually, this information can be useful in the theoretical design of drugs with favorable, improved specificity and activity    .
Targeting inflammation is a broad task, since many mediators are involved in onset of particular disease. Among these many mediators, free radicals are of great interest because of their major contribution in establishment of chronic inflammation and cancer. The well known immunosuppressive and anti-inflammatory drugs that are commercially available are mainly non-selective in their mechanism of action and also exhibit numerous side effects. The purpose of current review is to understand the new target site via targeting oxidative stress in terms of nitric oxide and reactive oxygen species at cellular level. This might work to develop new anti-inflammatory molecules with specific target. As mentioned above, inducible nitric oxide synthase and phagocytic NADPH oxidase can be focused so that specific pathologies can be targeted. Keeping this in mind, the potential of anti-NADPH oxidase and iNOS inhibitors, could serve as promising therapeutic intervention for chronic inflammatory disorders.
List of Abbreviations
AA: Arachidonic Acid
AP-1: Activating Protein-1
CGD: Chronic Granulomatous Disease
DPI: Diphenyl iodonium
ER: Endoplasmic Reticulum
FAD: Flavin adenine dinucleotide
FBS: Fetal Bovine Serum
FMLP: Formylated peptides
GAP: GTPase activating protein
GNEF: Guanine Nucleotide Exchange Factor
GPCR: Gq-coupled receptors
IBD: Irritable bowel syndrome
IFNR1: Interferon Receptor-1
IKK: Inhibitor of κB Kinase
iNOS: inducible nitric oxide synthase
IP3: Inositol Trisphosphate
IRAK: Interleukin-1 Receptor-Associated Kinase
IRF1: Interferon Response Factor-1
JAK: Janus Kinase family
LBP: LPS-Binding Protein
MyD88: Myeloid Differentiation Primary Response Gene88
NADPH: Nicotinamide adenine dinucleotide
NDKB: Nucleoside Diphosphate Kinase B
NF-κB: Nuclear Factor-Κb
nNOS: Neuronal nitric oxide synthase
NOS-2: Nitric Oxide Synthase
Nox: NADPH oxidase
NSAIDs: Non steroidalantiinflammatory drugs
ONOO: Peroxy nitrite
Phox: Phagocytic oxidase
PIP2: Phosphatidylinositol Bisphosphate
PKC: Protein kinase C
PLCβ: phospholipase C β
RhoGDI: Rho GDP dissociation inhibitor
ROS: Reactive oxygen species
SOD: Superoxide Dismutase
STAT: Signal Transducers and Activators of Transcription
TAB1: TAK1-Binding Protein-1
TLR: Toll like receptor
TNF-α: Tumor Necrosis Factor-α
TRAF6: TNF Receptor-Associated Factor-6