ABSTRACT Chotpoti is a popular street food in the major areas of Bangladesh. Street foods play an important role in people’s daily food options as well as their regular nutritional requirements are dependent on these foods, as their ever-growing busy schedule takes away the opportunity to eat of homemade food. Due to the expedient availability, these street foods are one of the primary food options for city peoples. The present study was undertaken to investigate the microbiological quality of Chotpoti sold by different street vendor at Savar area, Dhaka, Bangladesh. Microbial quality was assessed by total viable count (TVC), total coliform count (TCC); samples were inoculated into various selective media such as Nutrient agar, Eosin Methylene Blue (EMB) agar, Salmonella Shigella (SS) agar, Thiosulfate Citrate Bile Salts Sucrose (TCBS) agar and Mac Conkey’s agar. Gram negative bacteria were isolated and identified from the samples. The TVC in Chotpoti sample was ranged from 6 × 105 CFU/g to TNTC and TCC was ranged from 6 × 105 CFU/g to 3 × 106 CFU/g. Antibiotic sensitivity test showed that the isolates were sensitive to antibiotics such as antibiotic against Streptomycin, Chloramphenicol. Data of this study indicated that Chotpoti sold at street around Savar region harbor multidrug resistant food borne bacteria which might cause public health hazards.
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Happy, A. , Alam, M. , Mahmud, S. , Imran, M. , Rony, M. , Azim, M. , Islam, M. , Sarker, M. , Akter, P. , Mondol, G. , Hossain, T. , Rahman, M. , Islam, M. , Roy, A. , Das, S. , Ahmed, M. and Uddin, M. (2018) Isolation, Identification and Characterization of Gram Negative Bacteria from Popular Street Food (Chotpoti) at Savar Area, Dhaka, Bangladesh. Open Access Library Journal, 5, 1-11. doi: 10.4236/oalib.1104986.
 Simopoulos, A.P. (2011) Evolutionary Aspects of Diet: The Omega-6/Omega-3 Ratio and the Brain. Molecular Neurobiology, 44, 203-215. https://doi.org/10.1007/s12035-010-8162-0
 Ghosh, M., Wahi, S., Kumar, M. and Ganguli, A. (2007) Prevalence of Enterotoxigenic Staphylococcus aureus and Shigella spp. in Some Raw Street Vended Indian Foods. International Journal of En-vironmental Health Research, 17, 151-156. https://doi.org/10.1080/09603120701219204
 Al Mamun, M., Rahman, S.M.M. and Turin, T.C. (2013) Knowledge and Awareness of Children’s Food Safety among School-Based Street Food Vendors in Dhaka, Bangladesh. Foodborne Pathogens and Disease, 10, 323-330. https://doi.org/10.1089/fpd.2012.1283
 Kaufman, C.E., Rumbaugh Whitesell, N., Keane, E., Giago, C. and Sam, A. (2014) Effectiveness of Circle of Life, an HIV-Preventive Intervention for American Indian Middle School Youths: A Group Randomized Trial in a Northern Plains Tribe. American Journal of Public Healthh, 104, 106-112. https://doi.org/10.2105/AJPH.2013.301822
 Civitello, D.J., Forys, P., Johnson, A.P. and Hall, S.R. (2012) Chronic Contamination Decreases Disease Spread: A Daphnia-Fungus-Copper Case Study. Proceedings of the Royal Society B-Biological Sciences, 279, 3146-3153. https://doi.org/10.1098/rspb.2012.0684
 Higman, V.A., Varga, K., Aslimovska, L., Judge, P.J., Sperling, L.J., Rienstra, C.M. and Watts, A. (2011) The Conformation of Bacteriorhodopsin Loops in Purple Membranes Resolved by Solid-State MAS NMR Spectroscopy. Angewandte Chemie International Edition, 50, 8432-8435. https://doi.org/10.1002/anie.201100730
 Paterson, D.L. (2006) Resistance in Gram-Negative Bacteria: Enterobacteriaceae. American Journal of Infection Control, 34, 20-28. https://doi.org/10.1016/j.ajic.2006.05.238
 Fernandez-Fuentes, M.A., Elena, O.M., Hikmate, A., Ruben, P.P. and Antonio, G. (2014) Antimicrobial Resistance Determinants in Antibiotic and Bio-cideresistant Gram-Negative Bacteria from Organic Foods. Food Control, 37, 9-14. https://doi.org/10.1016/j.foodcont.2013.08.041
 Viswanathan, P. and Kaur, R. (2001) Prevalence and Growth of Pathogens on Salad Vegetables, Fruits and Sprouts. International Journal of Hygiene and Environmental Health, 20, 205-213. https://doi.org/10.1078/S1438-4639(04)70030-9
 Mondol, G.C., Alam, M.G., Ahmad, T., Sharif, I.H., Hossain, M.A. and Uddin, M.E. (2018) Prevalence of Antibiotic Resistant Staphylococcus aureus among Patients Who Come to Seek Treatment in a Hospital of Bangladesh. Clinical Biotechnology and Microbiology, 2, 451-455.
 Rahman, M.M., Rahman, M.H. and Ansary, N.P. (2014) Safety Issues of Street Foods in Bangladesh. Journal of Biological Sciences and Technology, 2, 21-32.
 Biswas, S., Parvez, M.A.K., Shafiquzzaman, M. and Rahman, M.N. (2010) Isolation and Characterization of E. coli in Ready-to-Eat Foods Vended in Islamic University, Kushtia. Journal of Bio-Science, 18, 99-103.
 Zaid, K., Jamal, H. and Razak, C.K.L. (2011) The Prevalence of Microbiological Contamination in Ready-to-Eat Food and Factors Affecting It in Melaka. Journal of Community Health, 17, 64-73.
 Medina, E., Romero, C., Brenes, M. and De Castro, A. (2007) Antimicrobial Activity of Olive Oil, Vinegar and Various Beverages against Foodborne Pathogens. Journal of Food Protection, 70, 1194-1199. https://doi.org/10.4315/0362-028X-70.5.1194
 Estrada-Garcia, T., Lopez-Sancedo, C., Zamarripa-Ayala, B., Thompson, M.R., Gutierrez-Cogco, L., Mancera-Martinez, A. and Escobar-Gutierrez, A. (2004) Prevalence of E. coli and Salmonella spp in Street-Vended Food of Open Markets (Tianguis) and General Hygienic and Trading Practices in Mexico City. Epidemiology & In-fection, 132, 1181-1184. https://doi.org/10.1017/S0950268804003036
 Aneja, K.R. (2003) Staining and Biochemical Techniques. In: Experiments in Microbiology, Plant Pathology and Biotechnology, 4th Edition, New Age International Ltd., New Del-hi.
 Worcman-Barninka, D., Destro, M.T., Fernandes, S.A. and Landgraf, M. (2001) Evaluation of Motility Enrichment on Modified Semi-Solid Rappaport-Vassiliadis Medium (MSRV) for the Detection of Salmonella in Foods. International Journal of Food Microbiology, 64, 387-393. https://doi.org/10.1016/S0168-1605(00)00484-0
 Das, A., Nagananda, G.S., Bhattacharya, S. and Bhardwaj, S. (2010) Microbiological Quality of Street-Vended Indian Chaats Sold in Bangalore. Journal of Biological Sciences, 10, 255-260. https://doi.org/10.3923/jbs.2010.255.260
 Lewis, J.E., Thompson, P., Rao, B. and Rajanna, B. (2006) Human Bacteria in Street Vended Fruit Juices: A Case Study of Visakhapatnam City, India. Internet Journal of Food Safety, 8, 35-38.
 Jouve, J.L., Aagaard-Hansen, J. and Aidara-Kane, A. (2010) Food Safety: Equity and Social Determinants. Equity, Social Determinants and Public Health Programmes, 8, 95.
 Nyenje, M.E., Odjadjare, C.E., Tanih, N.F., Green, E. and Ndip, R.N. (2012) Foodborne Pathogens Recovered from Ready-to-Eat Foods from Roadside Cafeterias and Retail Outlets in Alice, Eastern Cape Province, South Africa. International Journal of Environmental Research and Public Health, 9, 2608-2619. https://doi.org/10.3390/ijerph9082608
 Tambekar, D.H., Jaiswal, V.J., Dhanorkar, D.V., Gulhane, P.B. and Dudhane, M.N. (2008) Identification of Microbiological Hazards and Safety of Ready-to-Eat Food Vended in Streets of Amravati City, India. Journal of Applied Biosciences, 7, 195-201.