ABSTRACT In chickens, infectious bronchitis (IB) is a major respiratory disease. The respiratory system is the primary multiplication site of IB virus (IBV), a coronavirus, after which the virus is distributed to other organs. Poultry farms sustain considerable economic damage due to IB outbreaks in flocks, since IB causes a severe reduction in weight gain in chicks. In the present study, we produced the ostrich IgY against IBV by immunizing female ostriches with the IB viral antigens. The resultant purified IgY showed a strong neutralizing activity against IBV infection of cultured primary chick kidney cells. The infectivity of IBV was markedly inhibited in the trachea of chicks when ostrich IgY was injected intra-muscularly into newly hatched chicks prior to viral inhalation challenge at two weeks of age. Furthermore, the infection was strongly blocked in the tracheae when IgY was injected into chicks at one day and one week of age, with viral inhalation performed at three weeks of age. These findings suggest that the injection of ostrich IgY can help protect young chicks from IBV infections. In south Asian and African countries, broiler chicks are sent to poultry market around 30 days of age, so it is important to prevent IB outbreaks in very young flocks. We strongly believe that ostrich IgY will be a powerful weapon against IB infection in poultry farms on a wide scale and also hope that these findings will aid in the development of antibody vaccines for new type corona viruses, SARS-CoV and MERS-CoV.
Cite this paper
Tsukamoto, Y. , Nakano, Y. and Adachi, K. (2018) Protection against Infectious Bronchitis Virus, a Corona Virus Infection, Using Ostrich Antibodies. Health, 10, 1294-1308. doi: 10.4236/health.2018.1010100.
 Bacon, L.D., Hunter, D.B., Zhang, H.M., Brand, K. and Etches, R. (2004) Retrospective Evidence That the MHC (B Haplotype) of Chickens Influences Genetic Resistance to Attenuated Infectious Bronchitis Vaccine Strains in Chickens. Avian Pathology, 33, 605-609. https://doi.org/10.1080/03079450400013147
 Tsukamoto, Y., Kotani, T., Shiraishi, Y., Kawamura, H. and Sakuma, S. (1996) Epithelial Cell Proliferation of Collecting Ducts and Ureters in the Regenerating Process of Interstitial Nephritis Caused by Infectious Bronchitis Virus. Avian Pathology, 25, 95-102. https://doi.org/10.1080/03079459608419123
 Tsukamoto, Y., Taira, E., Kotani, T., Yamate, J., Wada, S., Takaha, N., Miki, N. and Sakuma, S. (1996) Involvement of Gicerin, a Cell Adhesion Molecule, in Tracheal Development and Regeneration. Cell Growth & Differentiation, 7, 1761-1767.
 Albassam, M.A., Winterfield, R.W. and Thacker, H.L. (1986) Comparison of the Nephropathogenicity of Four Strains of Infectious Bronchitis Virus. Avian Diseases, 30, 468-476. https://doi.org/10.2307/1590408
 Cavanagh, D., Ellis, M.M. and Cook, J.K.A. (1997) Relationship between Variation in the S1 Spike Protein of Infectious Bronchitis Virus and the Extent of Cross-Protection. Avian Pathology, 26, 63-74.
 Tsukamoto, Y., Matsumoto, T., Kotani, T., Taira, E., Takaha, N., Miki, N., Yamate, J. and Sakuma, S. (1997) The Expression of Gicerin, a Cell Adhesion Molecule, in Regenerating Process of Collecting Ducts and Ureters of the Chicken Kidney after the Nephrotrophic Strain of Infectious Bronchitis Virus Infection. Avian Pathology, 26, 245-255. https://doi.org/10.1080/03079459708419209
 Cavanagh, D., Mawditt, K., Britton, P. and Naylor, C.J. (1999) Longitudinal Field Studies of Infectious Bronchitis Virus and Avian Pneumovirus in Broilers Using Type-Specific Polymerase Chain Reactions. Avian Pathology, 28, 593-605.
 Ambali, A.G. and Jones, R.C. (1990) Early Pathogenesis in Chicks with an Enterotropic Strain of Infectious Bronchitis Virus. Avian Diseases, 34, 809-817.
 Bumstead, N., Huggins, M.B. and Cook, J.K.A. (1989) Genetic Differences in Susceptibility to a Mixture of Avian Infectious Bronchitis Virus and Escherichia coli. British Poultry Science, 30,39-48. https://doi.org/10.1080/00071668908417123
 Casais, R., Davies, M., Cavanagh, D. and Britton, P. (2005) Gene 5 of the Avian Coronavirus Infectious Bronchitis Virus Is Not Essential for Replication. Journal of Virology, 79, 8065-8078. https://doi.org/10.1128/JVI.79.13.8065-8078.2005
 Smith, H.W., Cook, J.K.A. and Parsell, Z.E. (1985) The Experimental Infection of Chickens with Mixtures of Infectious Bronchitis Virus and Escherichia coli. Journal of General Virology, 66, 777-786.
 Hofstad, M.S. and Yoder Jr., H.W. (1996) Avian Infectious Bronchitis: Virus Distribution in Tissues of Chicks. Avian Diseases, 10, 230-239.
 Gay, K. (2000) Infectious Bronchitis Virus Detection and Persistence in Experimentally Infected Chickens. M.Sc. Thesis, Cornell University, New York.
 Cook, J.K.A., Smith, H.W. and Huggins, M.B. (1986) Infectious Bronchitis Immunity: Its Study in Chickens Experimentally Infected with Mixtures of Infectious Bronchitis Virus and Escherichia coli. Journal of General Virology, 67, 1427-1434.
 De Wit, J.J., de Jong, M.C., Pijpers, A. and Verheijden, J.H.M. (1998) Transmission of Infectious Bronchitis Virus within Vaccinated and Unvaccinated Groups of Chickens. Avian Pathology, 27, 464-471.
 Lambrechts, C., Pensaert, M. and Ducatelle, R. (1993) Challenge Wxperiments to Evaluate Cross-Protection Induced at the Trachea and Kidney Level by Vaccine Strains and Belgian Nephropathogenic Isolates of Avian Infectious Bronchitis Virus. Avian Pathology, 22, 577-590. https://doi.org/10.1080/03079459308418945
 Pensaert, M. and Lambrechts, C. (1994) Vaccination of Chickens against a Belgian Nephropathogenic Strain of Infectious Bronchitis Virus B1648 Using Attenuated Homologous and Heterologous Strains. Avian Pathology, 23, 631-641.
 Hodgson, T., Casais, R., Dove, B., Britton, P. and Cavanagh, D. (2004) Recombinant Infectious Bronchitis Coronavirus Beaudette with the Spike Protein Gene of the Pathogenic M41 Strain Remains Attenuated but Induces Protective Immunity. Journal of Virology, 78, 13804-13811.
 Gross, M. and Speck, J. (1996) Avian Yolk Antibodies in Diagnosis and Research. Deutsche tierarztliche Wochenschrift, 103, 417-422.
 Schade, R., Pfister, C., Halatsch, R. and Henklein, P. (1991) Polyclonal IgY Antibodies from Chicken Egg Yolk. An Alternative to the Production of Mammalian IgG Type Antibodies in Rabbits. ATLA, 19, 403-419.
 Schade, R., Schniering, A. and Hlinak, A. (1992) Polyclonal Avian Antibodies Extracted from Egg Yolk as an Alternative to the Production of Antibodies in Mammals—A Review. ALTEX, 9, 43-56.
 Larsson, A., Balow, R.M., Lindahl, T.L. and Forsberg, P.O. (1993) Chicken Antibodies: Taking Advantage of Evolution—A Review. Poultry Science, 72, 1807-1812.
 Leslie, G.A. and Clem, L.W. (1969) Phylogeny of Immunoglobulin Structure and Function. III. Immunoglobulins of the Chicken. The Journal of Experimental Medicine, 130, 1337-1352. https://doi.org/10.1084/jem.130.6.1337
 Adachi, K., Hagimori, K., Kikuta, T. and Tsukamoto, Y. (2008) Involvement of SC1, a Cell Adhesion Molecule, in the Progression of Mammary Gland Tumors. Molecular Medicine Reports, 1, 219-224.
 Adachi, K., Takama, K., Ozaki, M., Fukuda, K., Endo, I., Yamamoto, R. and Tsukamoto, Y. (2008) Inhibition of H5N1 Avian Influenza Virus Infection by Ostrich Antibodies. Molecular Medicine Reports, 1, 2003-2009.
 Reed, L.J. and Muench, H. (1938) A Simple Method of Estimating Fifty Percent Endo-Points. American Journal of Hygiene, 27, 493-797.
 Ignjatovic, J. and Galli, L. (1994) The S1 Glycoprotein but Not the N or M Proteins of Avian Infectious Bronchitis Virus Induces Protection in Vaccinated Chickens. Archives of Virology, 138, 117-134. https://doi.org/10.1007/BF01310043
 Johnson, M.A., Pooley, C., Ignjatovic, J. and Tyack, S.G. (2003) A Recombinant Fowl Adenovirus Expressing the S1 Gene of Infectious Bronchitis Virus Protects against Challenge with Infectious Bronchitis Virus. Vaccine, 21, 2730-2736.
 Macnaughton, M.R., Hasony, H.J., Madge, M.H. and Reed, S.E. (1981) Antibody to Virus Components in Volunteers Experimentally Infected with Human Coronavirus 229E Group Viruses. Infection and Immunity, 31, 845-849.
 Song, C.S., Lee, T.J., Lee, C.W., Sung, H.W., Kim, J.H., Mo, I.P., Izumiya, Y., Jang, H.K. and Mikami, T. (1998) Induction of Protective Immunity in Chickens Vaccinated with Infectious Bronchitis Virus S1 Glycoprotein Expressed by a Recombinant Baculovirus. Journal of General Virology, 79, 719-723.
 Holmes, H.C. (1973) Neutralizing Antibody in Nasal Secretions of Chickens Following Administration of Avian Infectious Bronchitis Virus. Arch Gesamte Virusforsch, 43, 235-241. https://doi.org/10.1007/BF01250418
 Davelaar, F.G. and Kouwenhoven, B. (1976) Changes in the Harderian Gland of the Chicken Following Conjunctival and Intranasal Infection with Infectious Bronchitis Virus in One- and 20-Day Old Chickens. Avian Pathology, 5, 39-50.
 Rosenberger, J.K. and Gelb Jr., J. (1987) Response of Several Avian Respiratory Viruses as Affected by Infectious Bursal Disease Virus. Avian Diseases, 22, 95-105.
 Thompson, B.G., Mohammed, H., Bauman, B. and Naqi, S. (1997) Systemic and Local Antibody Responses to Infectious Bronchitis Virus in Infectious Bursal Disease Inoculated and Control Chickens. Avian Diseases, 41, 519-527.
 Gelb, J., Nix, W.A. and Gellman, S.D. (1998) Infectious Bronchitis Virus Antibodies in Tears and Their Relationship to Immunity. Avian Diseases, 42, 364-374.
 Chubb, R.C. (1974) The Effect of the Suppression of Circulating Antibody on Resistance to the Australian Avian Infectious Bronchitis Virus. Research in Veterinary Science, 17, 169-173.
 Cook, J.K.A., Davidson, T.F., Huggins, M.B. and McLaughlan, P.I. (1991) Effect of in Ovo Bursectomy on the Course of an Infectious Bronchitis Virus Infection in Line C White Leghorn Chickens. Archives of Virology, 118, 225-234.
 Klieve, A.V. and Cumming, R.B. (1988) Immunity and Cross-Protection to Nephritis Produced by Australian Infectious Bronchitis Viruses Used as Vaccines. Avian Pathology, 17, 829-839. https://doi.org/10.1080/03079458808436505
 Klieve, A.V. and Cumming, R.B. (1988) Infectious Bronchitis: Safety and Protection in Chickens with Maternal Antibody. Australian Veterinary Journal, 65, 396-397.
 Mockett, A.P.A., Cook, J.K.A. and Huggins, M.B. (1987) Maternally-Derived Antibody to Infectious Bronchitis Virus: Its Detection in Chick Trachea and Serum and Its Role in Protection. Avian Pathology, 16, 407-416.
 Yachida, S., Sugimori, G., Aoyama, S., Takahashi, N., Iritani, Y. and Katagiri, K. (1981) Effectiveness of Maternal Antibody against Challenge with Infectious Bronchitis Viruses. Avian Diseases, 25, 736-741. https://doi.org/10.2307/1590005
 Suarez, D.L. and Schultz-Cherry, S. (2000) Immunology of Avian Influenza Virus: A Review. Developmental & Comparative Immunology, 24, 269-283.
 Simmons, C.P., Bernasconi, N.L., Suguitan, J.A.L., Mills, K., Ward, J.M., Chau, N.V.V., Tinh, T., Hien, T.T., Sallusto, F., Ha, D.Q., Farrar, J., de-Jong, M.D., Lanzavecchia, A. and Subbarao, K. (2007) Prophylactic and Therapeutic Efficacy of Human Monoclonal Antibodies against H5N1 Influenza. PLoS Medicine, 4, 928-936.