ABSTRACT A microbial study was conducted from wastewater
soils of hospitals in Aizawl, Mizoram, India which were in existence for a
longtime. The isolated bacteria from the wastewater soils were found to be
mainly of Morganella morganii and Bacillus cereus and these isolated
bacteria were found to be very resistant to a wide range of antibiotics and
heavy metals. Antibiotics that were used for treating infections caused by
these bacteria like chloramphenicol and ciprofloxacin were also found to be
insensitive. The degree of resistance was also very high when compared to
earlier reports of antibiotic resistance observed in the corresponding
bacteria. Our results suggested that the high degree of resistance is probably
conferred by the continued exposure to antibiotics from hospital waste leading
to a selected population of highly antibiotics-resistant bacteria.
Cite this paper
Hauhnar, L. , Pachuau, L. and Lalhruaitluanga, H. (2018) Isolation and Characterization of Multi-Drug Resistant Bacteria from Hospital Wastewater Sites around the City of Aizawl, Mizoram. Advances in Bioscience and Biotechnology, 9, 311-321. doi: 10.4236/abb.2018.97020.
 Iversen, A., Kuhn, I., Franlin, A. and Molby, R. (2002) High Prevalence of Vancomycin Resistant Enterococci in Swedish Wastewater. Applied Environmental Microbiology, 68, 2838-2842. https://doi.org/10.1128/AEM.68.6.2838-2842.2002
 Wise, R., Hart, T., Cars, O., Streulens, M., Helmuth, R., Huovinen, P. and Sprenger, M. (1998) Antimicrobial Resistance Is a Major Threat to Public Health. British Medical Journal, 317, 609-610. https://doi.org/10.1136/bmj.317.7159.609
 Mazel, D. and Davies, J. (1999) Antibiotic Resistance in Microbes. Cellular and Molecular Life Sciences, 56, 746-752. https://doi.org/10.1007/s000180050021
 Sharpe, M. (2003) High on Pollution: Drugs as Environmental Contaminants. Journal of Environmental Monitoring, 5, 43-46.
 Kruse, H. (1999) Indirect Transfer of Antibiotic Resistance Genes to Man. Acta Veterinaria Scandinavica, 92, 59-65.
 Berger-Bachi, B. (2002) Resistance Mechanisms of Gram-Positive Bacteria. International Journal of Medical Microbiology, 292, 27-35.
 Moura, A., Pereira, C., Henriques, I. and Correia, A. (2011) Novel Gene Cassettes and Integrons in Antibiotic-Resistant Bacteria from Urban Wastewaters. Research in Microbiology. (Early On-Line)
 Wegener, H., Aarestrup, F., Gerner-Smidt, P. and Bager, F. (1999) Transfer of Resistant Bacteria from Animals to Man. Acta Veterinaria Scandinavica, 92, 51-58.
 Verlicchi, P., Galletti, A., Petrovic, M. and Barcelo, D. (2010) Hospital Effluents as a Source of Emerging Pollutants: An Overview of Micropollutants and Sustainable Treatment Options. Journal of Hydrology, 389, 416-428.
 Kummerer, K. (2010) Pharmaceuticals in the Environment: Sources, Fate, Effects and Risks. Environmental Science and Pollution Research, 17, 519-521.
 Ahemad, A. and Mulugeta Kibret, M. (2013) Recent Trends in Microbial Biosorption of Heavy Metals: A Review. Biochemistry and Molecular Biology, 1, 19-26.
 Bruins, M.R., Kapil, S. and Oehme, F.W. (2000) Microbial Resistance to Metals in the Environment. Ecotoxicology and Environmental Safety, 45, 198-207.
 Nath, S., Deb, B. and Sharma, I. (2012) Isolation and Characterization of Cadmium and Lead Resistant Bacteria. Global Advanced Research Journal of Microbiology, 1, 194-198.
 Yamina, B., Tahar, B., Lila, M., Hocine, H. and Laure, F.M. (2014) Study on Cadmium-Resistant Bacteria Isolated from Hospital Wastewaters. Advances in Biosciences and Biotechnology, 5, 718-726. https://doi.org/10.4236/abb.2014.58085
 Stokes, H.W. and Gillings, M.R. (2011) Geneflow, Mobile Genetic Elements and the Recruitment of Antibiotic Resistance Genes into Gram-Negative Pathogens. FEMS Microbiology Reviews, 35, 790-819.
 Hookoom, M. and Puchooa, D. (2013) Isolation and Identification of Heavy Metals Tolerant Bacteria from Industrial and Agricultural Areas in Mauritius. Current Research in Microbiology and Biotechnology, 1, 119-123.
 Vilain, S., Luo, Y., Hildreth, M. and Brozel, V. (2006) Analysis of the Life Cycle of the Soil Saprophyte Bacillus cereus in Liquid Soil Extract and in Soil. Applied and Environmental Microbiology, 72, 4970-4977.
 Liu, H., Zhu, J., Hu, Q. and Rao, X. (2016) Morganella morganii, a Non-Negligent Opportunistic Pathogen. International Journal of Infectious Diseases, 50, 10-17.
 Falagas, M.E., Kavvadia, P.K., Mantadakis, E., Kofteridis, D.P., Bliziotis, I.A., Saloustros, E., Maraki, S. and Samonis, G. (2006) Morganella morganii Infections in a General Tertiary Hospital. Infection, 34, 315-321.
 Farmer, J.J. (1995) Enterobacteriaceae: Introduction and Identification. In: Murray, P.R., Baron, E.J., Pfaller, M.A., Tenover, F.C. and Yolken, R.H., Eds., Manual of Clinical Microbiology, 6th Edition, American Society for Microbiology, Washington DC, 438-449.
 Rojas, L., Vinuesa, T., Tubau, F., Truchero, C., Benz, R. and Vinas, M. (2006) Integron Presence in a Multiresistant Morganella morganii Isolate. International Journal of Antimicrobial Agents, 27, 505-512.
 Bush, K., Jacoby, G.A. and Medeiros, A.A. (1995) A Functional Classification Scheme for β-Lactamases and Its Correlation with Molecular Structure. Antimicrobial Agents and Chemotherapy, 39, 1211-1233.
 DiPersio, J.R. and Krafczyk, T.L. (1980) In Vitro Activity of Netilmicin, Gentamicin, Tobramycin and Amikacin against Glucose Fermenting and Nonfermenting Bacteria. Chemotherapy, 26, 323-333. https://doi.org/10.1159/000237924
 Stock, I. and Wiedemann, B. (1998) Identification and Natural Antibiotic Susceptibility of Morganella morganii. Diagnostic Microbiology and Infectious Diseases, 30, 153-165. https://doi.org/10.1016/S0732-8893(97)00243-5
 Tilea, B., Szekely, E., Teches, S. and Tilea, I. (2015) Acute Community-Acquired Meningoencephalitis with Morganella morganii—A Case Report. Romanian Journal of Laboratory Medicine, 23, 333-340.
 Duport, C., Jobin, M. and Schmitt, P. (2016) Adaptation in Bacillus cereus: From Stress to Disease. Frontiers in Microbiology, 7, 1550.
 Kotiranta, A., Lounatmaa, K. and Haapasalo, M. (2000) Epidemiology and Pathogenesis of Bacillus cereus Infections. Microbes and Infection, 2, 189-198.
 Guinebretiere, M.H., Broussolle, V. and Nguyen-The, C. (2002) Enterotoxigenic Profiles of Food-Poisoning and Food-Borne Bacillus cereus Strains. Journal of Clinical Microbiology, 40, 3053-3056. https://doi.org/10.1128/JCM.40.8.3053-3056.2002
 Luna, A.V., King, D.S., Gulledge, J., Cannons, A.C., Amuso, P.T. and Cattani, J. (2007) Susceptibility of Bacillus anthracis, Bacillus cereus, Bacillus mycoides, Bacillus pseudomycoides and Bacillus thuringiensis to 24 Antimicrobials Using Sensititre Automated Microbroth Dilution and Etest Agar Gradient Diffusion Methods. Journal of Antimicrobial Chemotherapy, 60, 555-567.
 Kervick, G.N., Flynn Jr., H.W., Alfonso, E. and Miller, D. (1990) Antibiotic Therapy for Bacillus Species Infections. American Journal of Opthalmology, 110, 683-687.