To better understand the mechanism of sugar
signaling in rice cell, the suspension-cultured rice cells were transferred
from sucrose-containing (+S) to sucrose-free (-S) of MS culture medium, we
found that ribosomal RNAs (rRNAs) were degraded progressively. This suggests
that carbon, nitrogen, and phosphate were recycled in this process and the
reduction in cellular rRNAs might lead to decreased translation to save energy
in response to sugar starvation. Differential screening revealed that two
groups of genes, sugar-starvation-repressed (SSR) and
sugar-starvation-activated (SSA) genes, were regulated by sugar in an opposing
manner. Northern-blot analysis showed that two major hybridization signals of
0.8 and 1.9 kb were induced strongly under sugar starvation. The two
populations of genes corresponded with homologs of α-amylases (1.9 kb) and the glycine-rich proteins (GRPs) gene
family (0.8 kb), and all were SSA genes. Expression of GRP genes was strongly
induced in sugar-starved cells, which suggests that GRPs may help to protect
cells against nutritional stress. Treatment of +S and -S cells with the protein
kinase (PK) inhibitor staurosporine (St) and the serine/theronine
phosphoprotein phosphatases 1 (PP1) and 2A (PP2A) inhibitor okadaic acid (OA)
revealed that PP1 and PP2A (PPs) might be involved in increasing SSR gene
expression in +S cells, and that activation of the majority of the SSA genes in
-S cells might be due to PKs activity. These results suggested that PKs and PPs
might be involved in the sugar regulation of SSR and SSA gene expression. An
in-gel PK activity assay demonstrated that the activity of two classes of PKs
(50 and 66 kDa) may be induced rapidly after transfer of +S cells to -S medium.
Following transfer of -S cells to +S medium, a novel class of 38 kDa PK was
induced rapidly and showed high activity. The 38 kDa PK might play a role in
sugar sensing, and the 50 and 66 kDa PKs might play roles in signal sensing
under sugar starvation in rice cells. These results provide valuable
information on three classes of protein kinases that might play key roles in
sugar sensing and signaling in rice.
Cite this paper
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