ABSTRACT We investigated the relationship between muscle
inosine monophosphate (IMP) content and mRNA levels of ADSL, AMPD1, and ATIC in
Dapulian (DPL), Landrace × Dapulian (LDPL), and Duroc × Landrace × Dapulian
(DLDPL) hybridized pigs. Methods:The total RNA in longissimus dorsi was isolated from Dapulian (DPL), Landrace × Dapulian (LDPL) and Duroc ×
Landrace × Dapulian (DLDPL) hybridized pigs, weighed about 95.0 kg, n =
8/species. The internal genes with highest stability (YWHAZ and RPL4) were
chosen from 11 common internal genes using Quantitative real-time PCR (qPCR)
and geNorm software. The mRNA levels of ADSL, AMPD1 and ATIC genes were corrected with YWHAZ and RPL4 genes.
The muscular IMP content was determined by HPLC.The muscular IMP content in DPL was higher than that in LDPL and DLDPL,
25.00% (p<0.05) and 15.56% (p>0.05), respectively. The muscular mRNA level of ADSL gene in DPL and LDPL was higher than that in DLDPL, 24.14% and 12.07%, respectively (p<0.05). The muscular mRNA level of ATIC gene in DPL and LDPL was higher than that in DLDPL, 66.67% and 33.33%, respectively (p<0.05). The muscular mRNA level of AMPD1 gene in DPL and LDPL was higher than that in DLDPL, 14.49% and 33.26%, respectively. Furthermore,
the IMP content was positively correlated with the mRNA level of ADSL, AMPD1
and ATIC genes, respectively (p<0.05). The mRNA level of ADSL gene was highly related to that of AMPD1
and ATIC gene, respectively(p<0.01), while that of AMPD1 gene was not strongly correlated with that of
ATIC gene (p>0.05). The muscular mRNA level of
AMPD1, ADSL and ATIC genes and the muscular IMP content in DPL were
highest, followed by those in LDPL and DLDPL. The muscular IMP content was
positively correlated with the muscular mRNA level of ADSL, AMPD1 and ATIC
Cite this paper
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