NS  Vol.6 No.5 , March 2014
Role of Topical Application of Growth Factors on Periodontal Repair
ABSTRACT

Reconstruction of lost or injured periodontal tissues in such a way that both the original structures and their function are completely restored. Restoring of lost periodontal tissues involves a creation of new attachment, including the formation of a new periodontal ligament with its fibers inserted in newly formed cementum and alveolar bone. The purpose of this research is to illustrate the biological actions of topical application of growth factors TGF-β1, VEGF on periodontal cells and tissues, which are involved in periodontal regeneration. Fifty-four male Albino rats were subjected for non surgical operation of distal sides of both lower anterior teeth, right side was considered as experimental site treated with 1 μm of VEGF, TGF-β1, while left side treated with 1 μm of normal saline as a control one. All the samples were studied histologically and histometry at three interval periods 3, 7, 14 days postoperatively. The results demonstrated that the mean value of amount of new bone and the mean of the length of junctional epithelia is showed to be higher in VEGF group, while the mean of periodontal ligament width and mean of rate of bone maturation are reported to be higher in combination group. High mean value for the width of cellular cementum is recorded in TGF group. Conclusion: The present study suggested that growth factors application in injured periodontium have a role in periodontal healing and VEGF illustrates to be the most powerful one.


Cite this paper
Al-Hijazi, A. and Al-Mahammadawy, A. (2014) Role of Topical Application of Growth Factors on Periodontal Repair. Natural Science, 6, 351-361. doi: 10.4236/ns.2014.65036.
References
[1]   Zia, A., Khan, S., Bey, A., Gupta, N.D. and Mukhtar-Un-Nisar, S. (2011) Oral Biomarkers in the Diagnosis and Progression of Periodontal Diseases. Biology and Medicine, 3, 45-52.

[2]   Kaufman, E. and Lamster, I.B. (2000) Analysis of Saliva for Periodontal Diagnosis: A Review. Journal of Clinical Periodontology, 27, 453-465. http://dx.doi.org/10.1034/j.1600-051x.2000.027007453.x

[3]   Giannobile, W.V., Al-Shammari, K.F. and Sarment, D.P. (2003) Matrix Molecules and Growth Factors as Indicators of Periodontal Disease Activity. Periodontology, 31, 125-134.
http://dx.doi.org/10.1034/j.1600-0757.2003.03108.x

[4]   Gürkan, A., Emingil, G., Cinarcik, S. and Berdeli, A. (2006) Gingivalcrevicular Fluid Transforming Growth FactorBeta1 in Several Forms of Periodontal Disease. Archives of Oral Biology, 51, 906-912.
http://dx.doi.org/10.1016/j.archoralbio.2006.04.008

[5]   Goncalves, R.P., Damante, C.A., Moura Lima, F.L., Imbronito, A.V., Daumas Nunes, F. and Pustiglioni, F.E. (2009) Detection of MMP-2 and MMP-9 Salivary Levels in Patients with Chronic Periodontitis before and after Periodontal Treatment. Revista Odonto Ciência, 24, 264-269.

[6]   Pisoschi, C., Banita, M., Stanciulescu, C., Fusaru, A.M. and Gheorghita, M. (2009) Influence of Some Mediators of Extracellular Matrix Remodeling on Angiogenesis in Diabetic Gingival Overgrowth. FEBS Journal, 276, 217.

[7]   Keles, G.C., Cetinkaya, B.O., Eroglu, C., Simsek, S.B. and Kahraman, H. (2010) Vascular Endothelial Growth Factor Expression Levels of Gingiva in Gingivitis and Periodontitis Patients with/without Diabetes Mellitus. Inflammation Research, 59, 543-549. http://dx.doi.org/10.1007/s00011-010-0158-8

[8]   Buduneli, N. and Kinane, D.F. (2011) Host-Derived Diagnostic Markers Related to Soft Tissue Destruction and Bone Degradation in Periodontitis. Journal of Clinical Periodontology, 38, 85-105.
http://dx.doi.org/10.1111/j.1600-051X.2010.01670.x

[9]   Nemcovsky, C.E., Beny, L., Shanberger, S. and Feldman, H.S. (2004) Bone Apposition in Surgical Defects Following Orthodontic Movement. Journal of Periodontology, 75, 1021-1027.
http://dx.doi.org/10.1902/jop.2004.75.7.1013

[10]   Gerstner, E.R., Duda, D.G., di Tomaso, E., Ryg, P.A., Loeffler, J.S., Sorensen, A.G., Ivy, P., Jain, R.K. and Batchelor, T.T. (2009) VEGF Inhibitors in the Treatment of Cerebral Edema in Patients with Brain Cancer. Nature Reviews Clinical Oncology, 6, 229-236. http://dx.doi.org/10.1038/nrclinonc.2009.14

[11]   Shivdasani, A. and Ingham, Ph. (2003) Regulation of Stem Cell Maintenance and Transit Amplifying Cell Proliferation by TGF-β Signaling in Drosophila Spermatogenesis. Current Biology, 13, 2065-2072.
http://dx.doi.org/10.1016/j.cub.2003.10.063

[12]   Niida, S., Kaku, M., Amano, H., Yoshida, H., Kataoka, H. and Nishikawa, S. (1999) Vascular Endothelial Growth Factor Can Substitute for Macrophage Colony-Stimulating Factor in the Support of Osteoclastic Bone Resorption. Journal of Experimental Medicine, 190, 293-298. http://dx.doi.org/10.1084/jem.190.2.293

[13]   Olivos-Meza, A., Fitzsimmons, J., Casper, M. and Chen, Q. (2010) Pretreatment of Periosteum with TGF-β1 in Situ Enhances the Quality of Osteochondral Tissue Regenerated from Transplanted Periosteal Grafts in Adult Rabbits. Osteoarthritis Cartilage, 18, 1183-1191.
http://dx.doi.org/10.1016/j.joca.2010.06.003

[14]   Kasagi, Sh. and Chen, W. (2013) TGF-Beta1 on Osteoimmunology and the Bone Component Cells. Cell & Bioscience, 3, 2-7. http://dx.doi.org/10.1186/2045-3701-3-4

[15]   Tal, H. and Stahl, S.S. (1986) Periodontal Attachment Responses to Surgical Injury in the Cat. Removal of Buccal Bone with and without Placement of Foreign Body at Ligament Periphery. Journal of Clinical Periodontology, 13, 4551. http://dx.doi.org/10.1111/j.1600-051X.1986.tb01413.x

[16]   Polson, A., Caton, J., Polson, A.P., Nyman, S., Novak, J. and Reed, B. (1984) Periodontal Response after Tooth Movement into Infra-Bony Defects. Journal of Periodontology, 55, 197-202. http://dx.doi.org/10.1902/jop.1984.55.4.197

 
 
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