OJOG  Vol.4 No.1 , January 2014
Effect of daily supplementation of soy isoflavones on hot flashes and night sweats in African menopausal women

The aim of this study was to evaluate the effectiveness of a pharmaceutical grade isoflavone supplement (Inoclim?) 40 mg per capsule of soy extract rich in daidzin and genistin) on the number of hot flashes and night sweats in menopausal African women. This open, prospective, multicenter study involved 140 menopausal women with climacteric symptoms (i.e., a minimum of five hot flashes and/or moderate to severe night sweats per day). These patients received a daily 40-mg supplement of soy isoflavone contained in an Inoclim? capsule (Laboratoire Innotech International, France). The dose was increased to two capsules per day when there were more than five hot flashes or when the women could not sleep because of night sweats. The patients were followed up over a 4-month period and data were analyzed using EPI-InfoTM version 3.5.1. The outcome measure was fewer hot flashes and night sweats. The mean daily number of episodes of hot flashes was 5.7 at baseline and 1.8 at week 16, corresponding to a decrease of 68.4% (n = 93 patients at baseline and n = 66 at 16 weeks). The mean number of daily episodes of night sweats was 3.7 at baseline and 0.8 at week 16, corresponding to a decrease of 78.4% (n = 127 patients in the first week and n = 73 at 16 weeks). Over 80% of patients took a single one 40 mg capsule per day. The percentage of patients who adhered to the prescription was more than 95% during the 4-month follow-up period. These results confirm the effectiveness of a pharmaceutical grade isoflavone supplement (40 mg, rich in genistin and daidzin) on vasomotor symptoms in menopausal women.

Cite this paper
Mbu, R. , Abauleth, Y. , Koffi, A. , Keita, N. , Dolo, A. and Lankoande, J. (2014) Effect of daily supplementation of soy isoflavones on hot flashes and night sweats in African menopausal women. Open Journal of Obstetrics and Gynecology, 4, 42-46. doi: 10.4236/ojog.2014.41008.
[1]   Nelson, H.D. (2008) Menopause. Lancet, 371, 760-770. http://dx.doi.org/10.1016/S0140-6736 (08)60346-3

[2]   Gold, E.B., Colvin, A., Avis, N., Bromberger, J., Greendale, G.A., Powell, L., et al. (2006) Longitudinal analysis of the association between vasomotor symptoms and race/ ethnicity across the menopausal transition: Study of women’s health across the nation. American Journal of Public Health, 96, 1226-1235. http://dx.doi.org/10.2105/AJPH.2005.066936

[3]   Anolue, F.C., Dike, E., Adogu, P. and Ebirim, C. (2012) Women’s experience of menopause in rural communities in Orlu, Eastern Nigeria. International Journal of Gynecology & Obstetrics, 118, 31-33. http://dx.doi.org/10.1016/j.ijgo.2012.02.014

[4]   Ande, A.B., Omu, O.P., Ande, O.O. and Olagbuji, N.B. (2011) Features and perceptions of menopausal women in Benin City, Nigeria. Annals of African Medicine, 10, 300-304. http://dx.doi.org/10.4103/ 1596-3519.87048

[5]   Odiari, E.A. and Chambers, A.N. (2012) Perceptions, attitudes, and self-management of natural menopausal symptoms in ghanaian women. Health Care Women International, 33, 560-574.

[6]   Nkwo, P.O. (2009) Suboptimal management of severe menopausal symptoms by Nigerian Gynaecologists: A call for mandatory continuing medical education for physiccians. BMC Women’s Health, 9, 30. http://dx.doi.org/10.1186/1472-6874-9-30

[7]   Sidibe, E.H. (2005) Menopause in Africa. Annals of Endocrinology (Paris), 66, 105-107.

[8]   Rodstrom, K., Bengtsson, C., Lissner, L., Milsom, I., Sundh, V., Bjorkelund, C.A. (2002) longitudinal study of the treatment of hot flushes: The population study of women in Gothenburg during a quarter of a century. Menopause, 9, 156-161. http://dx.doi.org/10.1097/00042192-200205000-00003

[9]   Eden, J. (1998) Phytoestrogens and the menopause. Baillière’s Clinical Endocrinology and Metabolism, 12, 581587. http://dx.doi.org/10.1016/S0950-351X(98)80005-0

[10]   Ewies, A.A. (2002) Phytoestrogens in the management of the menopause: Up-to-date. Obstetrical & Gynecological Survey, 57, 306-313. http://dx.doi.org/10.1097/00006254-200205000-00023

[11]   Albertazzi, P. and Purdie, D. (2002) The nature and utility of the phytoestrogens: A review of the evidence. Maturitas, 42, 173-185. http://dx.doi.org/10.1016/S0378-5122(02)00024-5

[12]   Kuiper, G.G., Lemmen, J.G., Carlsson, B., Corton, J.C., Safe, S.H., van der Saag, P.T., van der Burg, B. and Gustafsson, J.A. (1998) Interaction of estrogenic chemicals and phytoestrogens with estrogen receptor beta. Endocrinology, 139, 4252-4263. http://dx.doi.org/10.1210/en.139.10.4252

[13]   Markiewicz, L., Garey, J., Adlercreutz, H. and Gurpide, E. (1993) In vitro bioassays of non-steroidal phytoestrogens. The Journal of Steroid Biochemistry and Molecular Biology, 45, 399-405.

[14]   Campagnoli, C., Abba, C., Ambroggio, S., Peris, C., Perona, M. and Sanseverino, P. (2005) Polyunsaturated fatty acids (PUFAs) might reduce hot flushes: An indication from two controlled trials on soy isoflavones alone and with a PUFA supplement. Maturitas, 51, 127-134.

[15]   Petri Nahas, E., Nahas Neto, J., De Luca, L., Traiman, P., Pontes, A. and Dalben, I. (2004) Benefits of soy germ isoflavones in postmenopausal women with contraindication for conventional hormone replacement therapy. Maturitas, 48, 372-380. http://dx.doi.org/10.1016/j.maturitas.2003.09.026

[16]   Penotti, M., Fabio, E., Modena, A.B., Rinaldi, M., Omodei, U. and Vigano, P. (2003) Effect of soy-derived isoflavones on hot flushes, endometrial thickness, and the pulsatility index of the uterine and cerebral arteries. Fertility and Sterility, 79, 1112-1117. http://dx.doi.org/10.1016/S0015-0282 (03)00158-4

[17]   Nikander, E., Kilkkinen, A., Metsa-Heikkila, M., Adlercreutz, H., Pietinen, P., Tiitinen, A., et al. (2003) A randomized placebo-controlled crossover trial with phytoestrogens in treatment of menopause in breast cancer patients. Obstetrics & Gynecology, 101, 1213-1220. http://dx.doi.org/10.1016/S0029-7844(03)00232-1

[18]   Faure, E.D., Chantre, P. and Mares, P. (2002) Effects of a standardized soy extract on hot flushes: A multicenter, double-blind, randomized, placebo-controlled study. Menopause, 9, 329-334. http://dx.doi.org/10.1097/00042192-200209000-00005

[19]   Anupongsanugool, E., Teekachunhatean, S., Rojanasthien, N., Pongsatha, S. and Sangdee, C. (2005) Pharmacokinetics of isoflavones, daidzein and genistein, after ingestion of soy beverage compared with soy extract capsules in postmenopausal Thai women. BMC Clinical Pharmacology, 5, 2.

[20]   Baber, R.J., Templeman, C., Morton, T., Kelly, G.E. and West, L. (1999) Randomized placebo-controlled trial of an isoflavone supplement and menopausal symptoms in women. Climacteric, 2, 85-92. http://dx.doi.org/10.3109/13697139909025571

[21]   Murkies, A.L., Lombard, C., Strauss, B.J., Wilcox, G., Burger, H.G. and Morton, M.S. (1995) Dietary flour supplementation decreases post-menopausal hot flushes: Effect of soy and wheat. Maturitas, 21, 189-195. http://dx.doi.org/10.1016/0378-5122(95)00899-V

[22]   Scambia, G., Mango, D., Signorile, P.G., Anselmi Angeli, R.A., Palena, C., Gallo, D., et al. (2000) Clinical effects of a standardized soy extract in postmenopausal women: a pilot study. Menopause, 7, 105-111. http://dx.doi.org/10.1097/00042192-200007020-00006

[23]   Washburn, S., Burke, G.L., Morgan, T. and Anthony, M. (1999) Effect of soy protein supplementation on serum lipoproteins, blood pressure, and menopausal symptoms in perimenopausal women. Menopause, 6, 7-13. http://dx.doi.org/10.1097/00042192-199906010-00004

[24]   Miksicek, R.J. (1994) Interaction of naturally occurring nonsteroidal estrogens with expressed recombinant human estrogen receptor. The Journal of Steroid Biochemistry and Molecular Biology, 49, 153-160. http://dx.doi.org/10.1016/0960-0760(94)90005-1

[25]   Kuiper, G.G., Carlsson, B., Grandien, K., Enmark, E., Haggblad, J., Nilsson, S., et al. (1997) Comparison of the ligand binding specificity and transcript tissue distribution of estrogen receptors alpha and beta. Endocrinology, 138, 863-870. http://dx.doi.org/10.1210/en.138.3.863

[26]   Upmalis, D.H., Lobo, R., Bradley, L., Warren, M., Cone, F.L. and Lamia, C.A. (2000) Vasomotor symptom relief by soy isoflavone extract tablets in postmenopausal women: A multicenter, double-blind, randomized, placebocontrolled study. Menopause, 7, 236-242. http://dx.doi.org/10.1097/000 42192-200007040-00005

[27]   Colacurci, N., Zarcone, R., Borrelli, A., De Franciscis, P., Fortunato, N., Cirillo, M., et al. (2004) Effects of soy isoflavones on menopausal neurovegetative symptoms. Minerva Ginecology, 56, 407-412.

[28]   Crisafulli, A., Marini, H., Bitto, A., Altavilla, D., Squadrito, G., Romeo, A., et al. (2004) Effects of genistein on hot flushes in early postmenopausal women: A randomized, double-blind EPTand placebo-controlled study. Menopause, 11, 400-404. http://dx.doi.org/10.1097/01.GME.0000109314. 11228.E5

[29]   Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail. Mars (2005) Sécurité et bénéfices des phyto-estrogènes apportés par l’alimentation-Recommandations. http://www.anses.fr/sites/default/files/documents/NUT-Sy-phytoestrogenes.pdf

[30]   Williamson-Hughes, P.S., Flickinger, B.D., Messina, M.J. and Empie, M.W. (2006) Isoflavone supplements containing predominantly genistein reduce hot flash symptoms: A critical review of published studies. Menopause, 13, 831-839. http://dx.doi.org/10.1097/01.gme.0000227330.49081. 9e

[31]   Howes, L.G., Howes, J.B. and Knight, D.C. (2006) Isoflavone therapy for menopausal flushes: A systematic review and meta-analysis. Maturitas, 55, 203-211. http://dx.doi.org/10.1016/j.maturitas. 2006.03.008

[32]   Albertazzi, P., Pansini, F., Bonaccorsi, G., Zanotti, L., Forini, E. and De Aloysio, D. (1998) The effect of dietary soy supplementation on hot flushes. Obstetrics & Gynecology, 91, 6-11. http://dx.doi.org/ 10.1016/S0029-7844(97)00597-8

[33]   Han, K.K., Soares Jr., J.M., Haidar, M.A., de Lima, G.R. and Baracat, E.C. (2002) Benefits of soy isoflavone therapeutic regimen on menopausal symptoms. Obstetrics & Gynecology, 99, 389-394. http://dx.doi.org/10.1016/S0029-7844(01)01744-6

[34]   NAMS (2011) 2011 isoflavones report. Menopause, 18, 732-753.

[35]   Taku, K. (2012) Extracted or synthesized soybean isoflavones reduce menopausal hot flash frequency and severity: Systematic review and meta-analysis of randomized controlled trials. Menopause, 19, 1-15. http://dx.doi.org/10.1097/gme.0b013e3182410159

[36]   Cheng, G. (2007) Isoflavone treatment for acute menopausal symptoms. Menopause, 14, 468-473. http://dx.doi.org/10.1097/GME.0b013e31802cc7d0

[37]   D’Anna, R. (2007) Effects of the phytoestrogen genistein on hot flushes, endometrium, and vaginal epithelium in postmenopausal women: A 1-year randomized, doubleblind, placebo-controlled study. Menopause, 14, 648-655. http://dx.doi.org/10.1097/01.gme.0000248708.60698.98

[38]   Evans, M. (2011) The effect of synthetic genistein on menopause symptom management in healthy postmenopausal women: A multi-center, randomized, placebo-controlled study. Maturitas, 68, 189-196. http://dx.doi.org/10.1016/j.maturitas.2010.11.012

[39]   Védrine, N. (2006) One-month exposure to soy isoflavones did not induce tha ability to produce equol in postmenopausal women. European Journal of Clinical Nutrition, 60, 1039-1045.

[40]   (2012) The 2012 hormone therapy position statement of the North American menopause society. Menopause, 19, 257-271.

[41]   Cassidy, A. (1999) Potential tissue selectivity of dietary phytoestrogens and estrogens. Current Opinion in Lipidology, 10, 47-52. http://dx.doi.org/10.1097/00041433-199902000-00009