ABB  Vol.4 No.11 , November 2013
Biochemical variability between two Egyptian Stenodactylus species (Reptilia: Gekkonidae) inhabiting North Sinai
ABSTRACT

Polyacrylamide gel electrophoreses for malate dehydrogenase (Mdh) and beta-esterase (β-Est) isoenzymes were conducted for biochemical differentiation between two Stenodactylus gekkonid species inhabiting North Sinai of Egypt. Total lipids and proteins of liver and muscle tissues in both species were also analyzed. A total of three Mdh isoforms were recorded in the analysis, in which the activity of Mdh-2 and Mdh-3 seemed to be higher in S. petrii than in S. sthenodactylus. This high activity could be supported by the significant increase in the total lipids and proteins in liver and muscle tissues of the species. It may thus be reasonable to suppose that S. petrii is more active, energetic and adaptable in the desert habitat than S. sthenodactylus. β-Est showed six fractions in S. petrii and only one fraction in S. sthenodactylus. It is therefore noticeable that β-Est is more highly expressed in S. petrii than in S. sthenodactylus.




Cite this paper
Kadry, M. and Amer, S. (2013) Biochemical variability between two Egyptian Stenodactylus species (Reptilia: Gekkonidae) inhabiting North Sinai. Advances in Bioscience and Biotechnology, 4, 974-978. doi: 10.4236/abb.2013.411129.
References
[1]   Ali, R.A.M. (2012) Genetic variation among nine Egyptian gecko species (Reptilia: Gekkonidae) based on RAPDPCR. Journal of Life Science, 9, 154-162.

[2]   Baha El Din, M. (1997) A new species of tarentola (Squamata: Gekkonidae) from the western desert of Egypt. African Journal of Herpetology, 46, 30-35.
http://dx.doi.org/10.1080/21564574.1997.9649973

[3]   Saleh, M.A. (1997) Amphibians and reptiles of Egypt. [Cairo]: Egyptian Environmental Affairs Agency.

[4]   Castiglia, R. (2004) First chromosomal analysis for the genus Lygodactylus (Gray, 1864): The karyotype of L. picturatus (Squamata, Gekkonidae, Gekkoninae). African Journal of Herpetology, 53, 95-97.
http://dx.doi.org/10.1080/21564574.2004.9635502

[5]   Kawai, A., Ishijima, J., Nishida, C., Kosaka, A., Ota, H., Kohno, S. and Matsuda, Y. (2009) The ZW sex chromosomes of Gekkohokouensis (Gekkonidae, Squamata) represent highly conserved homology with those of avian species. Chromosoma, 118, 43-51.
http://dx.doi.org/10.1007/s00412-008-0176-2

[6]   Amer, S.A.M. (1999) Morphometric, genetic and metabolic variations in relation to the taxonomic separation of gekkonids in Egypt (Gekkonidae: Reptilia). PhD Thesis, Cairo University, Egypt.

[7]   Macey, R., Ananjeva, B., Wang, Y. and Papenfuss, J. (2000) Phylogenetic relationships among Asian gekkonid lizards formerly of the genus Cyrtodactylus based on cladistic analyses of allozymic data: Monophyly of Cyr-topodion and Mediodactylus. Journal of Herpetology, 34, 258-265. http://dx.doi.org/10.2307/1565422

[8]   Qin, X., Liang, Y. and Huang, X. (2006) Isozymes analysis on different tissues from three different populations of Gekko gecko. Guangxi Science, 13, 310-316.

[9]   Carranza, S., Arnold, N., Mateo, A. and Geniez, P. (2002) Relationships and evolution of the North African geckos, Geckonia and Tarentola (Reptilia: Gekkonidae), based on mitochondrial and nuclear DNA sequences. Molecular Phylogenetics and Evolution, 23, 244-256.
http://dx.doi.org/10.1016/S1055-7903(02)00024-6

[10]   Han, D., Zhou, K. and Bauer, M. (2004) Phylogenetic relationships among gekkotan lizards inferred from Cmos nuclear DNA sequences and anew classification of the Gekkota. Biological Journal of Linnean Society, 83, 353-368.
http://dx.doi.org/10.1111/j.1095-8312.2004.00393.x

[11]   Vences, M., Wanke, S., Vieites, R., Branch, R., Glaw, F. and Meyer, A. (2004) Natural colonization or introductions Phylogeographical relationships and morphological differentiation of house geckos (Hemidactylus) from Madagascar. Biological Journal of Linnean Society, 83, 115-130. http://dx.doi.org/10.1111/j.1095-8312.2004.00370.x

[12]   Jesus, J., Brehm, A. and Harris, J. (2005) Phylogenetic relationships of Hemidactylus geckos from the Gulf of Guinea islands: Patterns of natural colonizations and anthropogenic introductions estimated from mitochondrial and nuclear DNA sequences. Molecular Phylogenetics and Evolution, 34, 480-485.
http://dx.doi.org/10.1016/j.ympev.2004.11.006

[13]   Rocha, S., Carretero, M. and Harris, J. (2005) Diversity and phylogenetic relationships of Hemidactylus geckos from the Comoro Islands. Molecular Phylogenetics and Evolution, 35, 292-299.
http://dx.doi.org/10.1016/j.ympev.2004.11.023

[14]   Qin, X., Liang, Y., Huang, X. and Pang, G. (2005) RAPD analysis on genetic divergence and phylogenesis of Gekko gecko from different areas. Chinese Journal of Zoology, 40, 14.

[15]   Carranza, S. and Arnold, N. (2006) Systematics, biogeography, and evolution of Hemidactylus geckos (Reptilia: Gekkonidae) elucidated using mitochondrial DNA sequences. Molecular Phylogenetics and Evolution, 38, 531-545. http://dx.doi.org/10.1016/j.ympev.2005.07.012

[16]   Bansal, R. and Karanth, P. (2010) Molecular phylogeny of Hemidactylus geckos (Squamata: Gekkonidae) of the Indian subcontinent reveals a unique Indian radiation and an Indian origin of Asian house geckos. Molecular Phylogenetics and Evolution, 57, 459-465.
http://dx.doi.org/10.1016/j.ympev.2010.06.008

[17]   Rato, C., Carranza, S., Pereira, A., Carretero, M. and Harris, J. (2010) Conflicting patterns of nucleotide diversity between mtDNA and nDNA in the Moorish gecko, Tarentola mauritanica. Molecular Phylogenetics and Evolution, 56, 962-971.
http://dx.doi.org/10.1016/j.ympev.2010.04.033

[18]   Busais, S. and Joger, U. (2011) Molecular phylogeny of the gecko genus Hemidactylus Oken, 1817 on the mainland of Yemen (Reptilia: Gekkonidae). Zoology in the Middle East, 53, 25-34.
http://dx.doi.org/10.1080/09397140.2011.10648859

[19]   Fujita, K. and Papenfuss, J. (2011) Molecular systematics of Stenodactylus (Gekkonidae), an Afro-Arabian gecko species complex. Molecular Phylogenetics and Evolution, 58, 71-75. http://dx.doi.org/10.1016/j.ympev.2010.10.014

[20]   Baha El Din, M. (2006) A guide to reptiles and amphibians of Egypt. Cairo: American University in Cairo Press.

[21]   Laganà, G., Bellocco, E., Mannucci, C., Leuzzi, U., Tellone, E., Kotyk1, A. and Galtieri, A. (2006) Enzymatic urea adaptation: Lactate and malate dehydrogenase in elasmobranchs. Physiological Research, 55, 675-688.

[22]   Lo, A.S.Y., Liew, C.T., Ngai, S.M., Tsui, S.K.W., Fung, K.P., Lee, C.Y. and Waye, M.M.Y. (2005) Developmental regulation and cellular distribution of human cytosolic malate dehydrogenase (MDH1). Journal of Cell Biochemistry, 94, 763-773.
http://dx.doi.org/10.1002/jcb.20343

[23]   Danis, P. and Farkas, R. (2009) Hormone-dependant and hormone-independant control of metabolic and developmental functionals of malate dehydrogenase. Endocrine Regulations, 43, 39-52.
http://dx.doi.org/10.4149/endo_2009_01_39

[24]   Shahjahan, R.M., Karim, A., Begum, R.A., Alam, M.S. and Begum, A. (2008) Tissue specific esterase isozyme banding pattern in Nile Tilapia (Oreochromis niloticus). University Journal of Zoology (Rajshahi University), 27, 1-5.

[25]   Stegemann, H., Afify, A.M.R. and Hussein, K.R.F. (1985) Cultivar identification of dates (Phoenix dactylifera) by protein patterns. Second international symposium of biochemical approaches to identification of cultivars, Brounschweig, Germany, 44.

[26]   Jonathan, F.W. and Wendel, N.F. (1990) Visualization and interpretation of plant isozymes. In: Soltis, D.E. and Soltis, P.S., Eds., Isozymes in Plant Biology, Champan and Hall, London, 5-45.

[27]   Scandaliojs, G. (1964) Tissue-specific isozyme variations in maize. Journal of Heredity, 55, 281-285.

[28]   ZÖllner, N. and Kirsch, K. (1962) Colorimetric method for determination of total lipids. Journal of Experimental Medicine, 135, 545-550.
http://dx.doi.org/10.1007/BF02045455

[29]   Gornall, A.G., Bardawill, C.J. and David, M.M. (1949) Determination of serum proteins by means of the biuret reaction. Journal of Biological Chemistry, 177, 751-766.

[30]   Allendorff, W. and Utter, F.M. (1978) Population genetics. In: Hoaran, W.S. and Randalal, D.J., Eds., Fish Physiology 8, Academic Press, New York, 407-454.

[31]   Al-Harbi, M.S. and Amer, S.A.M. (2012) Comparison of energy-related isoenzymes between production and rac- ing Arabian camels. Advances in Bioscience and Bio- technology, 3, 1124-1128.

 
 
Top